CRISPR Gene Knockout/gRNA Degisn

Ubigene developed a CRISPR Gene-Editing strategy designer - Red Cotton™ . Red Cotton™ is based on our actual successfully gene-editing cases for more than 3000 genes, and the related parameters for more than 800 cell lines, combining with the technical advantages of Ubigene-developed CRISPR-U™ for gene KO. Red Cotton™ includes various one-stop tools covering from strategy design to KO validation, and functions such as gRNA design, primer design, vector design, sequence alignment, etc. Ubigene has comprehensively constructed a powerful gene KO system. No matter you are expert or freshly started researchers, Red Cotton™ can make your gene-KO experiment easier!

Red Cotton™ CRISPR Gene Editing Designer

3 ready-to-use KO cell line strategies | 3000 successful cases of gene KO | Parameters of more than 800 cell line

Red Cotton™ CRISPR gene editing system (Red Cotton™ system), developed by Ubigene, is an automatic system with thousands of gene-editing data, bioinformatics and IT technology. You only need to input your target gene and cell line information, and you will get the following information in a minute: 3sets of ready-to-use KO cell line strategies; More than 800 cell line related parameters; 3000 successful cases of gene knockout.

Tools for gene editing

Sequencing data analysis | Primer design | DNA reverse complement | DNA translation | Sequence alignment | Sequence dot-plot | GC content

Red Cotton™ includes various one-stop gene-editing tools, covering from strategy design to KO validation, and functions such as gRNA design, primer design, analysis of sequencing results, etc.

CRISPR-U™

Exclusive innovation, 10 times more efficient in gene-editing.

CRISPR-U™ (based on CRISPR/Cas9 technology), developed by Ubigene, is more efficient than general CRISPR/Cas9 in double-strand breaking, and CRISPR-U™ can greatly improve the efficiency of homologous recombination, easily achieve knockout (KO), point mutation (PM) and knockin (KI) in vitro and in vivo. With CRISPR-U™, Ubigene has successfully modified genes on more than 100 cell lines.

CRISPR/Cas9 recognizes the target sequence with gRNA, and guides Cas9 endonuclease to cut the upstream of PAM, resulting in the double-strand break (DSB) of DNA the target site. To repair the DSB, the cell uses its own DNA repair mechanism to add or delete or replace pieces of DNA sequences via Homology Directed Repair (HDR) or Non-Homologous End Joining (NHEJ).

Red Cotton™ gRNA plasmid bank

Sign-up Red Cotton™ now and over 600 types of gene plasmids are ready for you!

Ubigene has set-up our gRNA plasmid bank, 10000 in-stock plasmids available. Only $80, 3-5 business days!

Ubigene Biosciences

Make genome editing easier

Ubigene Biosciences is co-founded by biological academics and elites from China, the United States, and France. We are located in Guangzhou Science City, which serves as a global center for high technology and innovation. Ubigene Biosciences has 2000㎡ office areas and laboratories, involving genome editing, cell biology technology, and zebrafish research. We provide products and services for plasmids, viruses, cells, and zebrafish. We aim to provide customers with better gene-editing tools for cell or animal research.

Make genome editing easier is the goal of Ubigene. We developed CRISPR-U™ (based on CRISPR/Cas9 technology) which is more efficient than general CRISPR/Cas9 in double-strand breaking, and CRISPR-U™ can greatly improve the efficiency of homologous recombination, easily achieve knockout (KO), point mutation (PM) and knockin (KI) in vitro and in vivo. With CRISPR-U™, Ubigene has successfully edit genes on more than 100 cell lines.

Pragmatism, efficiency, integrity are our business principles. We are committed to providing the best services and products to every customer who chooses us.

Genome Editing Platform
Focusing on the Application of CRISPR-U™ and CRISPR-B™ Gene Editing Technology
1. Provides various types of gene-editing vectors for different species.
2. Provides different virus packaging services, including lentiviruses, adenoviruses and adeno-associated viruses.
3. Provides high-quality services for gene knockout, point mutation, knockin cell lines and microbe.

Cell Biology Platform Cell Biology Platform
Focusing on primary cells
1. Provides over 400 types of primary cells.
2. Provides culture strategies and related products for different cell types.
3. Provides cell biology-related services such as cell isolation, extraction and validation.

Cell gene knockout scheme
Success Cases Cell Solution
Digestive SystemDigestive System
Human Colon Cancer Cell Line HCT 116
Human Colon Cancer Cell Line SW480
Human Colon Cancer Cell Line SW620
Human Colon Cancer Cell Line HCT 116
Human Colon Cancer Cell Line SW480
Human Colon Cancer Cell Line SW620
Human Colon Cancer Cell Line HT-29
Human Colon Cancer Cell Line LoVo
Human colon carcinoma cell line RKO
Human caucasian colon adenocarcinoma cell line COLO 205
Murine colorectal carcinoma cell line CT26.WT
Human colon adenocarcinoma cell line DLD-1
Human colorectal adenocarcinoma cell line NCI-H716
Human colorectal adenocarcinoma cell line Caco-2
Human colon carcinoma cell line T84
Human liver cancer cell line Hep G2
Human hepatocellular carcinoma cell line HuH-7
Human Hepatocellular Carcinoma Cell Line SNU-387
Human hepatobiliary cancer cell line RBE
Human Gastric Cancer Cell Line HGC-27
Human gastric cancer cell line AGS
Human Esophageal Squamous Carcinoma Cell Line KYSE-150
Human Esophageal Squamous Carcinoma Cell Line KYSE-30
Human Colon Cancer Cell Line HCT 116
Human Colon Cancer Cell Line SW480
Human Colon Cancer Cell Line SW620
Human Colon Cancer Cell Line HT-29
Human Colon Cancer Cell Line LoVo
Human colon carcinoma cell line RKO
Human caucasian colon adenocarcinoma cell line COLO 205
Murine colorectal carcinoma cell line CT26.WT
Human colon adenocarcinoma cell line DLD-1
Human colorectal adenocarcinoma cell line NCI-H716
Human colorectal adenocarcinoma cell line Caco-2
Human colon carcinoma cell line T84
Human liver cancer cell line Hep G2
Human hepatocellular carcinoma cell line HuH-7
Human Hepatocellular Carcinoma Cell Line SNU-387
Human hepatobiliary cancer cell line RBE
Human Gastric Cancer Cell Line HGC-27
Human gastric cancer cell line AGS
Human Esophageal Squamous Carcinoma Cell Line KYSE-150
Human Esophageal Squamous Carcinoma Cell Line KYSE-30
Endocrine SystemEndocrine System
Human Breast Cancer Cell Line MDA-MB-231
Human Breast Cancer Cell Line MDA-MB-468
Human Breast Cancer Cell Line MDA-MB-453
Human Breast Cancer Cell Line MDA-MB-436
Rat Breast Cancer Cell Line 4T1
Human Pancreatic Carcinoma Cell Line PANC-1
Human Breast Adenocarcinoma Cell Line SK-BR-3
Human Metastatic Pancreatic Adenocarcinoma Cell Line AsPC-1
Human Pancreatic Carcinoma Cell Line MIA PaCa-2
Human prostate cancer cell line PC-3
Human Prostate Cancer Cell Line VCaP
Mouse Acinar Pancreatic Cell Line 266-6
Human pancreatic cancer cell line BxPC-3
Human Breast Cancer Cell Line MDA-MB-231
Human Breast Cancer Cell Line MDA-MB-468
Human Breast Cancer Cell Line MDA-MB-453
Human Breast Cancer Cell Line MDA-MB-436
Rat Breast Cancer Cell Line 4T1
Human Pancreatic Carcinoma Cell Line PANC-1
Human Breast Adenocarcinoma Cell Line SK-BR-3
Human Metastatic Pancreatic Adenocarcinoma Cell Line AsPC-1
Human Pancreatic Carcinoma Cell Line MIA PaCa-2
Human prostate cancer cell line PC-3
Human Prostate Cancer Cell Line VCaP
Mouse Acinar Pancreatic Cell Line 266-6
Human pancreatic cancer cell line BxPC-3
Respiratory SystemRespiratory System
Human Lung Cancer Cell Line A549
Human Lung Cancer Cell Line Calu-1
Human lung squamous cell carcinoma cell line SK-MES-1
Human Lung Squamous Cell Carcinoma Cell Line NCI-H226
Human Non-small Cell Lung Carcinoma Cell Line NCI-H1299
Human Non-small Cell Lung Carcinoma Cell Line HCC827
Human Bronchial Epithelial Cell Line BEAS-2B
Human Bronchial Epithelial Cell Line 16HBE
Human hypopharyngeal carcinoma cell line FaDu
Human Lung Cancer Cell Line A549
Human Lung Cancer Cell Line Calu-1
Human lung squamous cell carcinoma cell line SK-MES-1
Human Lung Squamous Cell Carcinoma Cell Line NCI-H226
Human Non-small Cell Lung Carcinoma Cell Line NCI-H1299
Human Non-small Cell Lung Carcinoma Cell Line HCC827
Human Bronchial Epithelial Cell Line BEAS-2B
Human Bronchial Epithelial Cell Line 16HBE
Human hypopharyngeal carcinoma cell line FaDu
Blood and Lymphatic SystemBlood and Lymphatic System
Human Monocytic Cell Line THP-1
Rat Basophil Leukemia Cell Line RBL-2H3
Human leukemia cell line HL-60
Human myelogenous leukemia cell line K-562
Human caucasian histiocytic lymphoma cell line U-937
Human Acute Non-B Non-T Lymphocytic Leukemia Cell Line Reh
Human Monocytic Cell Line THP-1
Rat Basophil Leukemia Cell Line RBL-2H3
Human leukemia cell line HL-60
Human myelogenous leukemia cell line K-562
Human caucasian histiocytic lymphoma cell line U-937
Human Acute Non-B Non-T Lymphocytic Leukemia Cell Line Reh
Urinary SystemUrinary System
Human bladder carcinoma cell line TCCSUP
Human bladder carcinoma cell line 5637
Human Bladder Transitional Cell Carcinoma Cell Line T24
Human Embryonic Kidney Cell Line 293T
Human prostate cancer cell line 22RV1
Human bladder carcinoma cell line TCCSUP
Human bladder carcinoma cell line 5637
Human Bladder Transitional Cell Carcinoma Cell Line T24
Human Embryonic Kidney Cell Line 293T
Human prostate cancer cell line 22RV1
Reproductive SystemReproductive System
Human Cervical Carcinoma Cell Line HeLa
Human Cervical Carcinoma Cell Line HeLa 229
Human Cervical Squamous Cell Line SiHa
Human Cervical Carcinoma Cell Line HeLa
Human Cervical Carcinoma Cell Line HeLa 229
Human Cervical Squamous Cell Line SiHa
Chinese Hamster Ovary Cell Line CHO-K1
Human Ovarian Cancer Cell Line SK-OV-3
Human Ovarian Cancer Cell Line OVCAR3
Mouse Pituitary Cell Line Lbetat2
Mouse Testicular Stromal Cell Line TM3
Circulatory SystemCirculatory System
Mouse Myoblast Cell Line C2C12
Brain and Nervous SystemBrain and Nervous System
Rat Glioblastoma Cell Line C6
Mouse Glioblastoma Cell Line GL261
Human glioblastoma cell line U-87 MG
Mouse Anterior Parietal Bone Cell Line MC3T3-E1 Subclone 14
Mouse Hippocampal Neuron Cell Line HT22
Human Neuroblastoma Cell Line SK-N-SH
Mouse neuroblastoma cell line Neuro-2a
Human Glioblastoma Cell Line U251
Rat Glioblastoma Cell Line C6
Mouse Glioblastoma Cell Line GL261
Human glioblastoma cell line U-87 MG
Mouse Anterior Parietal Bone Cell Line MC3T3-E1 Subclone 14
Mouse Hippocampal Neuron Cell Line HT22
Skeleton, Articulus, Soft Tissue, Derma SystemSkeleton, Articulus, Soft Tissue, Derma System
Human Osteosarcoma Cell Line MG-63
Human bone osteosarcoma epithelial cell line U-2 OS
Human fibrosarcoma cell line HT-1080
Human malignant melanoma cell line A-375
Human Melanoma Cell Line M14
Murine melanoma cell line B16-F10
Human Osteosarcoma Cell Line MG-63
Human bone osteosarcoma epithelial cell line U-2 OS
Human fibrosarcoma cell line HT-1080
Human malignant melanoma cell line A-375
Human Melanoma Cell Line M14
Murine melanoma cell line B16-F10
Ocular, Otolaryngologic and Oral SystemOcular, Otolaryngologic and Oral System
Human Nasopharyngeal Carcinoma Cell Line CNE2Z
Human Nasopharyngeal Carcinoma Cell Line CNE2Z
Microbial gene knockout design strategy

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