CRISPR-U™ Gene Knockout Cell Line Strategy

CAV1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CAV1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CAV1
Gene id 857
Organism Homo sapiens
Official full symbol caveolin 1
Gene type protein-coding
Also known as BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21
Summary The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.
Genomic regions Chromosome 7
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CAV1-205 ENST00000405348.6 2652 147aa Protein coding CCDS55156 Q03135-2 - TSL:5, GENCODE basic, APPRIS ALT1,
CAV1-202 ENST00000393467.1 2628 147aa Protein coding CCDS55156 Q03135-2 - TSL:1, GENCODE basic, APPRIS ALT1,
CAV1-201 ENST00000341049.7 2456 178aa Protein coding CCDS5767 Q03135-1 NM_001753.5 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
CAV1-203 ENST00000393468.1 845 147aa Protein coding CCDS55156 Q03135-2 - TSL:1, GENCODE basic, APPRIS ALT1,
CAV1-209 ENST00000614113.5 1130 115aa Protein coding - Q03135-2 - TSL:1, GENCODE basic,
CAV1-204 ENST00000393470.1 598 167aa Protein coding - E9PCT5 - TSL:4, GENCODE basic,
CAV1-207 ENST00000456473.5 557 138aa Protein coding - C9JKI3 - CDS 3' incomplete, TSL:4,
CAV1-206 ENST00000451122.5 1653 86aa Nonsense mediated decay - F8WDM7 - TSL:1,
CAV1-208 ENST00000489856.1 575 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CAV1
This KO Strategy loading
Red Cotton™ Notes Gene CAV1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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