CRISPR-U™ Gene Knockout Cell Line Strategy

EMD Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EMD Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EMD
Gene id 2010
Organism Homo sapiens
Official full symbol emerin
Gene type protein-coding
Also known as EDMD, LEMD5, STA
Summary Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.
Genomic regions Chromosome X
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EMD-202 ENST00000369842.9 1281 254aa Protein coding CCDS14745 P50402 NM_000117.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
EMD-201 ENST00000369835.3 966 219aa Protein coding - Q5HY57 - TSL:3, GENCODE basic,
EMD-203 ENST00000428228.5 946 21aa Nonsense mediated decay - F8WEQ1 - TSL:3,
EMD-208 ENST00000492448.1 832 No protein Processed transcript - - - TSL:2,
EMD-207 ENST00000486738.5 1284 No protein Retained intron - - - TSL:3,
EMD-205 ENST00000471965.1 800 No protein Retained intron - - - TSL:2,
EMD-206 ENST00000485261.1 792 No protein Retained intron - - - TSL:3,
EMD-209 ENST00000494443.5 765 No protein Retained intron - - - TSL:2,
EMD-204 ENST00000468294.5 501 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EMD
This KO Strategy loading
Red Cotton™ Notes Gene EMD had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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