CRISPR-U™ Gene Knockout Cell Line
Strategy
EMD Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EMD Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EMD |
| Gene id | 2010 |
| Organism | Homo sapiens |
| Official full symbol | emerin |
| Gene type | protein-coding |
| Also known as | EDMD, LEMD5, STA |
| Summary | Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EMD-202 | ENST00000369842.9 | 1281 | 254aa | Protein coding | CCDS14745 | P50402 | NM_000117.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EMD-201 | ENST00000369835.3 | 966 | 219aa | Protein coding | - | Q5HY57 | - | TSL:3, GENCODE basic, |
| EMD-203 | ENST00000428228.5 | 946 | 21aa | Nonsense mediated decay | - | F8WEQ1 | - | TSL:3, |
| EMD-208 | ENST00000492448.1 | 832 | No protein | Processed transcript | - | - | - | TSL:2, |
| EMD-207 | ENST00000486738.5 | 1284 | No protein | Retained intron | - | - | - | TSL:3, |
| EMD-205 | ENST00000471965.1 | 800 | No protein | Retained intron | - | - | - | TSL:2, |
| EMD-206 | ENST00000485261.1 | 792 | No protein | Retained intron | - | - | - | TSL:3, |
| EMD-209 | ENST00000494443.5 | 765 | No protein | Retained intron | - | - | - | TSL:2, |
| EMD-204 | ENST00000468294.5 | 501 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EMD |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EMD had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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