CRISPR-U™ Gene Knockout Cell Line Strategy

IKBKG Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IKBKG Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IKBKG
Gene id 8517
Organism Homo sapiens
Official full symbol inhibitor of nuclear factor kappa B kinase regulatory subunit gamma
Gene type protein-coding
Also known as AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9
Summary This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome.
Genomic regions Chromosome X
Strategy Summary
This gene has 13 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IKBKG-207 ENST00000611071.4 2103 419aa Protein coding CCDS14757 Q9Y6K9-1 - TSL:1, GENCODE basic, APPRIS P4,
IKBKG-214 ENST00000618670.4 2069 487aa Protein coding CCDS48196 Q9Y6K9-2 - TSL:1, GENCODE basic, APPRIS ALT2,
IKBKG-206 ENST00000594239.6 1973 419aa Protein coding CCDS14757 Q9Y6K9-1 NM_001099857.5 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
IKBKG-212 ENST00000617207.4 1949 418aa Protein coding CCDS83513 A0A087X1B1 - TSL:3, GENCODE basic, APPRIS ALT2,
IKBKG-208 ENST00000611176.4 1654 320aa Protein coding CCDS48197 Q9Y6K9-3 - TSL:2, GENCODE basic,
IKBKG-215 ENST00000619941.4 1463 412aa Protein coding - A0A087WUW6 - TSL:5, GENCODE basic, APPRIS ALT2,
IKBKG-211 ENST00000615874.4 1460 411aa Protein coding - A0A087X0G7 - TSL:5, GENCODE basic, APPRIS ALT2,
IKBKG-203 ENST00000440286.5 960 250aa Protein coding - C9JN51 - CDS 3' incomplete, TSL:5,
IKBKG-201 ENST00000413620.5 892 195aa Protein coding - C9J2V2 - CDS 3' incomplete, TSL:5,
IKBKG-202 ENST00000422680.5 841 172aa Protein coding - C9JH59 - CDS 3' incomplete, TSL:2,
IKBKG-204 ENST00000445622.5 693 138aa Protein coding - C9JCG6 - CDS 3' incomplete, TSL:5,
IKBKG-205 ENST00000492469.2 552 181aa Protein coding - A0A087X1K7 - CDS 5' incomplete, TSL:5,
IKBKG-210 ENST00000615186.4 488 115aa Protein coding - A0A087WV30 - CDS 3' incomplete, TSL:5,
IKBKG-209 ENST00000612051.1 1972 48aa Nonsense mediated decay - A0A087WWQ9 - TSL:1,
IKBKG-213 ENST00000617838.1 285 No protein Processed transcript - - - TSL:1,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IKBKG
This KO Strategy loading
Red Cotton™ Notes Gene IKBKG had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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