CRISPR-U™ Gene Knockout Cell Line
Strategy
IKBKG Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IKBKG Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IKBKG |
| Gene id | 8517 |
| Organism | Homo sapiens |
| Official full symbol | inhibitor of nuclear factor kappa B kinase regulatory subunit gamma |
| Gene type | protein-coding |
| Also known as | AMCBX1, EDAID1, FIP-3, FIP3, Fip3p, IKK-gamma, IKKAP1, IKKG, IMD33, IP, IP1, IP2, IPD2, NEMO, ZC2HC9 |
| Summary | This gene encodes the regulatory subunit of the inhibitor of kappaB kinase (IKK) complex, which activates NF-kappaB resulting in activation of genes involved in inflammation, immunity, cell survival, and other pathways. Mutations in this gene result in incontinentia pigmenti, hypohidrotic ectodermal dysplasia, and several other types of immunodeficiencies. A pseudogene highly similar to this locus is located in an adjacent region of the X chromosome. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 13 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IKBKG-207 | ENST00000611071.4 | 2103 | 419aa | Protein coding | CCDS14757 | Q9Y6K9-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| IKBKG-214 | ENST00000618670.4 | 2069 | 487aa | Protein coding | CCDS48196 | Q9Y6K9-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| IKBKG-206 | ENST00000594239.6 | 1973 | 419aa | Protein coding | CCDS14757 | Q9Y6K9-1 | NM_001099857.5 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| IKBKG-212 | ENST00000617207.4 | 1949 | 418aa | Protein coding | CCDS83513 | A0A087X1B1 | - | TSL:3, GENCODE basic, APPRIS ALT2, |
| IKBKG-208 | ENST00000611176.4 | 1654 | 320aa | Protein coding | CCDS48197 | Q9Y6K9-3 | - | TSL:2, GENCODE basic, |
| IKBKG-215 | ENST00000619941.4 | 1463 | 412aa | Protein coding | - | A0A087WUW6 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| IKBKG-211 | ENST00000615874.4 | 1460 | 411aa | Protein coding | - | A0A087X0G7 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| IKBKG-203 | ENST00000440286.5 | 960 | 250aa | Protein coding | - | C9JN51 | - | CDS 3' incomplete, TSL:5, |
| IKBKG-201 | ENST00000413620.5 | 892 | 195aa | Protein coding | - | C9J2V2 | - | CDS 3' incomplete, TSL:5, |
| IKBKG-202 | ENST00000422680.5 | 841 | 172aa | Protein coding | - | C9JH59 | - | CDS 3' incomplete, TSL:2, |
| IKBKG-204 | ENST00000445622.5 | 693 | 138aa | Protein coding | - | C9JCG6 | - | CDS 3' incomplete, TSL:5, |
| IKBKG-205 | ENST00000492469.2 | 552 | 181aa | Protein coding | - | A0A087X1K7 | - | CDS 5' incomplete, TSL:5, |
| IKBKG-210 | ENST00000615186.4 | 488 | 115aa | Protein coding | - | A0A087WV30 | - | CDS 3' incomplete, TSL:5, |
| IKBKG-209 | ENST00000612051.1 | 1972 | 48aa | Nonsense mediated decay | - | A0A087WWQ9 | - | TSL:1, |
| IKBKG-213 | ENST00000617838.1 | 285 | No protein | Processed transcript | - | - | - | TSL:1, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IKBKG |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IKBKG had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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