CRISPR-U™ Gene Knockout Cell Line Strategy

EZR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EZR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EZR
Gene id 7430
Organism Homo sapiens
Official full symbol ezrin
Gene type protein-coding
Also known as CVIL, CVL, HEL-S-105, VIL2
Summary The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EZR-201 ENST00000337147.11 3068 586aa Protein coding CCDS5258 P15311 - TSL:1, GENCODE basic, APPRIS P2,
EZR-202 ENST00000367075.4 3052 586aa Protein coding CCDS5258 P15311 NM_001111077.2 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
EZR-203 ENST00000392177.8 2933 586aa Protein coding - E7EQR4 - TSL:5, GENCODE basic, APPRIS ALT1,
EZR-204 ENST00000476189.1 956 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EZR
This KO Strategy loading
Red Cotton™ Notes Gene EZR had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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