CRISPR-U™ Gene Knockout Cell Line
Strategy
EZR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EZR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EZR |
| Gene id | 7430 |
| Organism | Homo sapiens |
| Official full symbol | ezrin |
| Gene type | protein-coding |
| Also known as | CVIL, CVL, HEL-S-105, VIL2 |
| Summary | The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EZR-201 | ENST00000337147.11 | 3068 | 586aa | Protein coding | CCDS5258 | P15311 | - | TSL:1, GENCODE basic, APPRIS P2, |
| EZR-202 | ENST00000367075.4 | 3052 | 586aa | Protein coding | CCDS5258 | P15311 | NM_001111077.2 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| EZR-203 | ENST00000392177.8 | 2933 | 586aa | Protein coding | - | E7EQR4 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| EZR-204 | ENST00000476189.1 | 956 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EZR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EZR had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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