CRISPR-U™ Gene Knockout Cell Line Strategy

CDK4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDK4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDK4
Gene id 1019
Organism Homo sapiens
Official full symbol cyclin dependent kinase 4
Gene type protein-coding
Also known as CMM3, PSK-J3
Summary The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported.
Genomic regions Chromosome 12
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDK4-201 ENST00000257904.11 1865 303aa Protein coding CCDS8953 P11802-1 NM_000075.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDK4-202 ENST00000312990.10 1650 111aa Protein coding - Q96BE9 - TSL:1, GENCODE basic,
CDK4-204 ENST00000547281.5 825 186aa Protein coding - F8VZZ0 - CDS 3' incomplete, TSL:3,
CDK4-203 ENST00000546489.5 776 208aa Protein coding - F8VZ51 - CDS 3' incomplete, TSL:5,
CDK4-209 ENST00000551800.5 773 132aa Protein coding - F8VXD2 - CDS 3' incomplete, TSL:5,
CDK4-211 ENST00000552254.5 769 203aa Protein coding - F8VWX7 - CDS 3' incomplete, TSL:2,
CDK4-212 ENST00000552388.1 694 170aa Protein coding - F8VYH9 - CDS 3' incomplete, TSL:3,
CDK4-206 ENST00000549606.5 558 40aa Protein coding - F8VZ13 - TSL:2, GENCODE basic,
CDK4-214 ENST00000552862.1 447 115aa Protein coding - F8W1L8 - CDS 3' incomplete, TSL:3,
CDK4-205 ENST00000547853.1 228 2aa Protein coding - - - CDS 3' incomplete, TSL:3,
CDK4-215 ENST00000553237.5 952 74aa Nonsense mediated decay - F8VYY1 - TSL:2,
CDK4-207 ENST00000550419.5 919 201aa Nonsense mediated decay - F8VTV8 - TSL:3,
CDK4-210 ENST00000551888.5 636 No protein Processed transcript - - - TSL:2,
CDK4-208 ENST00000551706.1 984 No protein Retained intron - - - TSL:1,
CDK4-213 ENST00000552713.5 685 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDK4
This KO Strategy loading
Red Cotton™ Notes Gene CDK4 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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