CRISPR-U™ Gene Knockout Cell Line
Strategy
CDK4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDK4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDK4 |
| Gene id | 1019 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase 4 |
| Gene type | protein-coding |
| Also known as | CMM3, PSK-J3 |
| Summary | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDK4-201 | ENST00000257904.11 | 1865 | 303aa | Protein coding | CCDS8953 | P11802-1 | NM_000075.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CDK4-202 | ENST00000312990.10 | 1650 | 111aa | Protein coding | - | Q96BE9 | - | TSL:1, GENCODE basic, |
| CDK4-204 | ENST00000547281.5 | 825 | 186aa | Protein coding | - | F8VZZ0 | - | CDS 3' incomplete, TSL:3, |
| CDK4-203 | ENST00000546489.5 | 776 | 208aa | Protein coding | - | F8VZ51 | - | CDS 3' incomplete, TSL:5, |
| CDK4-209 | ENST00000551800.5 | 773 | 132aa | Protein coding | - | F8VXD2 | - | CDS 3' incomplete, TSL:5, |
| CDK4-211 | ENST00000552254.5 | 769 | 203aa | Protein coding | - | F8VWX7 | - | CDS 3' incomplete, TSL:2, |
| CDK4-212 | ENST00000552388.1 | 694 | 170aa | Protein coding | - | F8VYH9 | - | CDS 3' incomplete, TSL:3, |
| CDK4-206 | ENST00000549606.5 | 558 | 40aa | Protein coding | - | F8VZ13 | - | TSL:2, GENCODE basic, |
| CDK4-214 | ENST00000552862.1 | 447 | 115aa | Protein coding | - | F8W1L8 | - | CDS 3' incomplete, TSL:3, |
| CDK4-205 | ENST00000547853.1 | 228 | 2aa | Protein coding | - | - | - | CDS 3' incomplete, TSL:3, |
| CDK4-215 | ENST00000553237.5 | 952 | 74aa | Nonsense mediated decay | - | F8VYY1 | - | TSL:2, |
| CDK4-207 | ENST00000550419.5 | 919 | 201aa | Nonsense mediated decay | - | F8VTV8 | - | TSL:3, |
| CDK4-210 | ENST00000551888.5 | 636 | No protein | Processed transcript | - | - | - | TSL:2, |
| CDK4-208 | ENST00000551706.1 | 984 | No protein | Retained intron | - | - | - | TSL:1, |
| CDK4-213 | ENST00000552713.5 | 685 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDK4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDK4 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: