CRISPR-U™ Gene Knockout Cell Line
Strategy
FTL Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FTL Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FTL |
| Gene id | 2512 |
| Organism | Homo sapiens |
| Official full symbol | ferritin light chain |
| Gene type | protein-coding |
| Also known as | LFTD, NBIA3 |
| Summary | This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FTL-201 | ENST00000331825.11 | 871 | 175aa | Protein coding | CCDS33070 | A0A384MDR3 P02792 | NM_000146.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FTL |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FTL had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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