CRISPR-U™ Gene Knockout Cell Line
Strategy
TSPO Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human TSPO Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | TSPO |
| Gene id | 706 |
| Organism | Homo sapiens |
| Official full symbol | translocator protein |
| Gene type | protein-coding |
| Also known as | BPBS, BZRP, DBI, IBP, MBR, PBR, PBS, PKBS, PTBR, mDRC, pk18 |
| Summary | Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| TSPO-203 | ENST00000396265.4 | 1075 | 169aa | Protein coding | CCDS33661 | P30536-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| TSPO-201 | ENST00000329563.8 | 955 | 169aa | Protein coding | CCDS33661 | P30536-1 | - | TSL:3, GENCODE basic, APPRIS P1, |
| TSPO-202 | ENST00000337554.8 | 836 | 169aa | Protein coding | CCDS33661 | P30536-1 | NM_000714.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| TSPO-204 | ENST00000428336.5 | 672 | 107aa | Protein coding | - | B1AH87 | - | CDS 3' incomplete, TSL:5, |
| TSPO-206 | ENST00000583777.5 | 636 | 65aa | Protein coding | - | J3QLD3 | - | TSL:1, GENCODE basic, |
| TSPO-205 | ENST00000472378.1 | 495 | No protein | Retained intron | - | - | - | TSL:2, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | TSPO |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene TSPO had been KO in hct116 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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