CRISPR-U™ Gene Knockout Cell Line Strategy

TSPO Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human TSPO Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol TSPO
Gene id 706
Organism Homo sapiens
Official full symbol translocator protein
Gene type protein-coding
Also known as BPBS, BZRP, DBI, IBP, MBR, PBR, PBS, PKBS, PTBR, mDRC, pk18
Summary Present mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternatively spliced transcript variants have been reported; one of the variants lacks an internal exon and is considered non-coding, and the other variants encode the same protein.
Genomic regions Chromosome 22
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
TSPO-203 ENST00000396265.4 1075 169aa Protein coding CCDS33661 P30536-1 - TSL:5, GENCODE basic, APPRIS P1,
TSPO-201 ENST00000329563.8 955 169aa Protein coding CCDS33661 P30536-1 - TSL:3, GENCODE basic, APPRIS P1,
TSPO-202 ENST00000337554.8 836 169aa Protein coding CCDS33661 P30536-1 NM_000714.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
TSPO-204 ENST00000428336.5 672 107aa Protein coding - B1AH87 - CDS 3' incomplete, TSL:5,
TSPO-206 ENST00000583777.5 636 65aa Protein coding - J3QLD3 - TSL:1, GENCODE basic,
TSPO-205 ENST00000472378.1 495 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene TSPO
This KO Strategy loading
Red Cotton™ Notes Gene TSPO had been KO in hct116 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×