CRISPR-U™ Gene Knockout Cell Line
Strategy
EGFR Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human EGFR Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | EGFR |
| Gene id | 1956 |
| Organism | Homo sapiens |
| Official full symbol | epidermal growth factor receptor |
| Gene type | protein-coding |
| Also known as | ERBB, ERBB1, HER1, NISBD2, PIG61, mENA |
| Summary | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| EGFR-201 | ENST00000275493.7 | 9905 | 1210aa | Protein coding | CCDS5514 | P00533-1 | NM_005228.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| EGFR-206 | ENST00000454757.6 | 5464 | 1165aa | Protein coding | CCDS87506 | E9PFD7 | - | TSL:1, GENCODE basic, |
| EGFR-207 | ENST00000455089.5 | 3844 | 1091aa | Protein coding | CCDS87507 | Q504U8 | - | TSL:1, GENCODE basic, |
| EGFR-203 | ENST00000344576.6 | 2864 | 705aa | Protein coding | CCDS5515 | P00533-3 | - | TSL:1, GENCODE basic, |
| EGFR-202 | ENST00000342916.7 | 2239 | 628aa | Protein coding | CCDS5516 | P00533-4 | - | TSL:1, GENCODE basic, |
| EGFR-204 | ENST00000420316.6 | 1570 | 405aa | Protein coding | CCDS47587 | P00533-2 | - | TSL:1, GENCODE basic, |
| EGFR-205 | ENST00000450046.1 | 691 | 128aa | Protein coding | - | C9JYS6 | - | CDS 3' incomplete, TSL:4, |
| EGFR-209 | ENST00000463948.1 | 561 | No protein | Processed transcript | - | - | - | TSL:3, |
| EGFR-208 | ENST00000459688.1 | 452 | No protein | Processed transcript | - | - | - | TSL:3, |
| EGFR-210 | ENST00000485503.1 | 665 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | EGFR |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene EGFR had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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