CRISPR-U™ Gene Knockout Cell Line Strategy

EGFR Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human EGFR Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol EGFR
Gene id 1956
Organism Homo sapiens
Official full symbol epidermal growth factor receptor
Gene type protein-coding
Also known as ERBB, ERBB1, HER1, NISBD2, PIG61, mENA
Summary The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2).
Genomic regions Chromosome 7
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
EGFR-201 ENST00000275493.7 9905 1210aa Protein coding CCDS5514 P00533-1 NM_005228.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
EGFR-206 ENST00000454757.6 5464 1165aa Protein coding CCDS87506 E9PFD7 - TSL:1, GENCODE basic,
EGFR-207 ENST00000455089.5 3844 1091aa Protein coding CCDS87507 Q504U8 - TSL:1, GENCODE basic,
EGFR-203 ENST00000344576.6 2864 705aa Protein coding CCDS5515 P00533-3 - TSL:1, GENCODE basic,
EGFR-202 ENST00000342916.7 2239 628aa Protein coding CCDS5516 P00533-4 - TSL:1, GENCODE basic,
EGFR-204 ENST00000420316.6 1570 405aa Protein coding CCDS47587 P00533-2 - TSL:1, GENCODE basic,
EGFR-205 ENST00000450046.1 691 128aa Protein coding - C9JYS6 - CDS 3' incomplete, TSL:4,
EGFR-209 ENST00000463948.1 561 No protein Processed transcript - - - TSL:3,
EGFR-208 ENST00000459688.1 452 No protein Processed transcript - - - TSL:3,
EGFR-210 ENST00000485503.1 665 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene EGFR
This KO Strategy loading
Red Cotton™ Notes Gene EGFR had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×