CRISPR-U™ Gene Knockout Cell Line
Strategy
ATG16L1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATG16L1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATG16L1 |
| Gene id | 55054 |
| Organism | Homo sapiens |
| Official full symbol | autophagy related 16 like 1 |
| Gene type | protein-coding |
| Also known as | APG16L, ATG16A, ATG16L, IBD10, WDR30 |
| Summary | The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATG16L1-204 | ENST00000392018.1 | 3313 | 624aa | Protein coding | CCDS86927 | E7EVC7 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| ATG16L1-203 | ENST00000392017.9 | 3298 | 607aa | Protein coding | CCDS2503 | Q676U5-1 | NM_030803.7 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| ATG16L1-205 | ENST00000392020.8 | 3213 | 588aa | Protein coding | CCDS2502 | Q676U5-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| ATG16L1-201 | ENST00000347464.9 | 2915 | 444aa | Protein coding | CCDS54438 | Q676U5-5 | - | TSL:1, GENCODE basic, |
| ATG16L1-202 | ENST00000373525.9 | 2767 | 428aa | Protein coding | - | Q676U5-4 | - | TSL:5, GENCODE basic, |
| ATG16L1-210 | ENST00000444735.5 | 691 | 177aa | Protein coding | - | C9JK97 | - | CDS 3' incomplete, TSL:5, |
| ATG16L1-207 | ENST00000417017.5 | 684 | 187aa | Protein coding | - | C9J1B2 | - | CDS 3' incomplete, TSL:5, |
| ATG16L1-208 | ENST00000419681.5 | 644 | 164aa | Protein coding | - | C9JAY7 | - | CDS 3' incomplete, TSL:5, |
| ATG16L1-209 | ENST00000431917.5 | 589 | 55aa | Protein coding | - | C9J8C6 | - | CDS 3' incomplete, TSL:5, |
| ATG16L1-206 | ENST00000392021.7 | 3301 | 38aa | Nonsense mediated decay | - | F8WAF9 | - | TSL:1, |
| ATG16L1-218 | ENST00000498620.5 | 740 | No protein | Processed transcript | - | - | - | TSL:5, |
| ATG16L1-211 | ENST00000464645.5 | 560 | No protein | Processed transcript | - | - | - | TSL:4, |
| ATG16L1-214 | ENST00000474148.5 | 4208 | No protein | Retained intron | - | - | - | TSL:2, |
| ATG16L1-215 | ENST00000479942.5 | 3559 | No protein | Retained intron | - | - | - | TSL:1, |
| ATG16L1-212 | ENST00000472242.1 | 911 | No protein | Retained intron | - | - | - | TSL:4, |
| ATG16L1-213 | ENST00000473865.1 | 805 | No protein | Retained intron | - | - | - | TSL:2, |
| ATG16L1-217 | ENST00000492298.5 | 591 | No protein | Retained intron | - | - | - | TSL:5, |
| ATG16L1-216 | ENST00000485623.5 | 568 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATG16L1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATG16L1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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