CRISPR-U™ Gene Knockout Cell Line Strategy

ATG16L1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ATG16L1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATG16L1
Gene id 55054
Organism Homo sapiens
Official full symbol autophagy related 16 like 1
Gene type protein-coding
Also known as APG16L, ATG16A, ATG16L, IBD10, WDR30
Summary The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATG16L1-204 ENST00000392018.1 3313 624aa Protein coding CCDS86927 E7EVC7 - TSL:5, GENCODE basic, APPRIS ALT1,
ATG16L1-203 ENST00000392017.9 3298 607aa Protein coding CCDS2503 Q676U5-1 NM_030803.7 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
ATG16L1-205 ENST00000392020.8 3213 588aa Protein coding CCDS2502 Q676U5-2 - TSL:1, GENCODE basic, APPRIS ALT1,
ATG16L1-201 ENST00000347464.9 2915 444aa Protein coding CCDS54438 Q676U5-5 - TSL:1, GENCODE basic,
ATG16L1-202 ENST00000373525.9 2767 428aa Protein coding - Q676U5-4 - TSL:5, GENCODE basic,
ATG16L1-210 ENST00000444735.5 691 177aa Protein coding - C9JK97 - CDS 3' incomplete, TSL:5,
ATG16L1-207 ENST00000417017.5 684 187aa Protein coding - C9J1B2 - CDS 3' incomplete, TSL:5,
ATG16L1-208 ENST00000419681.5 644 164aa Protein coding - C9JAY7 - CDS 3' incomplete, TSL:5,
ATG16L1-209 ENST00000431917.5 589 55aa Protein coding - C9J8C6 - CDS 3' incomplete, TSL:5,
ATG16L1-206 ENST00000392021.7 3301 38aa Nonsense mediated decay - F8WAF9 - TSL:1,
ATG16L1-218 ENST00000498620.5 740 No protein Processed transcript - - - TSL:5,
ATG16L1-211 ENST00000464645.5 560 No protein Processed transcript - - - TSL:4,
ATG16L1-214 ENST00000474148.5 4208 No protein Retained intron - - - TSL:2,
ATG16L1-215 ENST00000479942.5 3559 No protein Retained intron - - - TSL:1,
ATG16L1-212 ENST00000472242.1 911 No protein Retained intron - - - TSL:4,
ATG16L1-213 ENST00000473865.1 805 No protein Retained intron - - - TSL:2,
ATG16L1-217 ENST00000492298.5 591 No protein Retained intron - - - TSL:5,
ATG16L1-216 ENST00000485623.5 568 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATG16L1
This KO Strategy loading
Red Cotton™ Notes Gene ATG16L1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

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