CRISPR-U™ Gene Knockout Cell Line Strategy

NDUFA13 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NDUFA13 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NDUFA13
Gene id 51079
Organism Homo sapiens
Official full symbol NADH:ubiquinone oxidoreductase subunit A13
Gene type protein-coding
Also known as B16.6, CDA016, CGI-39, GRIM-19, GRIM19, MC1DN28
Summary This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined.
Genomic regions Chromosome 19
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NDUFA13-204 ENST00000507754.9 521 144aa Protein coding CCDS12404 Q9P0J0-1 NM_015965.7 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NDUFA13-203 ENST00000503283.5 825 120aa Protein coding - B4DEZ3 - TSL:2, GENCODE basic,
NDUFA13-201 ENST00000428459.6 576 150aa Protein coding - K7EJE1 - TSL:2, GENCODE basic,
NDUFA13-202 ENST00000502506.6 1166 107aa Nonsense mediated decay - K7EP87 - TSL:2,
NDUFA13-207 ENST00000606722.1 502 75aa Nonsense mediated decay - U3KQP3 - CDS 5' incomplete, TSL:3,
NDUFA13-206 ENST00000511584.2 640 No protein Retained intron - - - TSL:2,
NDUFA13-205 ENST00000511180.4 593 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NDUFA13
This KO Strategy loading
Red Cotton™ Notes Gene NDUFA13 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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