CRISPR-U™ Gene Knockout Cell Line
Strategy
NDUFA13 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NDUFA13 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NDUFA13 |
| Gene id | 51079 |
| Organism | Homo sapiens |
| Official full symbol | NADH:ubiquinone oxidoreductase subunit A13 |
| Gene type | protein-coding |
| Also known as | B16.6, CDA016, CGI-39, GRIM-19, GRIM19, MC1DN28 |
| Summary | This gene encodes a subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which functions in the transfer of electrons from NADH to the respiratory chain. The protein is required for complex I assembly and electron transfer activity. The protein binds the signal transducers and activators of transcription 3 (STAT3) transcription factor, and can function as a tumor suppressor. The human protein purified from mitochondria migrates at approximately 16 kDa. Transcripts originating from an upstream promoter and capable of expressing a protein with a longer N-terminus have been found, but their biological validity has not been determined. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NDUFA13-204 | ENST00000507754.9 | 521 | 144aa | Protein coding | CCDS12404 | Q9P0J0-1 | NM_015965.7 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NDUFA13-203 | ENST00000503283.5 | 825 | 120aa | Protein coding | - | B4DEZ3 | - | TSL:2, GENCODE basic, |
| NDUFA13-201 | ENST00000428459.6 | 576 | 150aa | Protein coding | - | K7EJE1 | - | TSL:2, GENCODE basic, |
| NDUFA13-202 | ENST00000502506.6 | 1166 | 107aa | Nonsense mediated decay | - | K7EP87 | - | TSL:2, |
| NDUFA13-207 | ENST00000606722.1 | 502 | 75aa | Nonsense mediated decay | - | U3KQP3 | - | CDS 5' incomplete, TSL:3, |
| NDUFA13-206 | ENST00000511584.2 | 640 | No protein | Retained intron | - | - | - | TSL:2, |
| NDUFA13-205 | ENST00000511180.4 | 593 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NDUFA13 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NDUFA13 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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