CRISPR-U™ Gene Knockout Cell Line
Strategy
LOXL2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LOXL2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LOXL2 |
| Gene id | 4017 |
| Organism | Homo sapiens |
| Official full symbol | lysyl oxidase like 2 |
| Gene type | protein-coding |
| Also known as | LOR, LOR2, WS9-14 |
| Summary | This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LOXL2-201 | ENST00000389131.8 | 3721 | 774aa | Protein coding | CCDS34864 | Q9Y4K0 | NM_002318.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LOXL2-214 | ENST00000524144.5 | 975 | 258aa | Protein coding | - | E5RFY0 | - | CDS 3' incomplete, TSL:5, |
| LOXL2-209 | ENST00000520871.1 | 892 | 218aa | Protein coding | - | E5RHH3 | - | CDS 3' incomplete, TSL:2, |
| LOXL2-205 | ENST00000519243.1 | 763 | 95aa | Protein coding | - | E5RFE2 | - | CDS 3' incomplete, TSL:2, |
| LOXL2-215 | ENST00000524168.1 | 746 | 139aa | Protein coding | - | E5RI22 | - | CDS 3' incomplete, TSL:3, |
| LOXL2-207 | ENST00000520349.1 | 620 | 207aa | Protein coding | - | H0YAR1 | - | CDS 5' and 3' incomplete, TSL:3, |
| LOXL2-202 | ENST00000518083.5 | 588 | 151aa | Protein coding | - | E5RJL2 | - | CDS 3' incomplete, TSL:4, |
| LOXL2-212 | ENST00000523833.2 | 559 | 101aa | Protein coding | - | R4GMS2 | - | CDS 3' incomplete, TSL:5, |
| LOXL2-204 | ENST00000518878.5 | 689 | 67aa | Nonsense mediated decay | - | H0YAP6 | - | CDS 5' incomplete, TSL:3, |
| LOXL2-203 | ENST00000518472.1 | 725 | No protein | Processed transcript | - | - | - | TSL:3, |
| LOXL2-206 | ENST00000519809.5 | 571 | No protein | Processed transcript | - | - | - | TSL:4, |
| LOXL2-210 | ENST00000520925.1 | 965 | No protein | Retained intron | - | - | - | TSL:2, |
| LOXL2-211 | ENST00000522446.5 | 665 | No protein | Retained intron | - | - | - | TSL:3, |
| LOXL2-208 | ENST00000520617.1 | 502 | No protein | Retained intron | - | - | - | TSL:2, |
| LOXL2-213 | ENST00000524075.1 | 419 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LOXL2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LOXL2 had been KO in mcf7 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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