CRISPR-U™ Gene Knockout Cell Line Strategy

LOXL2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LOXL2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LOXL2
Gene id 4017
Organism Homo sapiens
Official full symbol lysyl oxidase like 2
Gene type protein-coding
Also known as LOR, LOR2, WS9-14
Summary This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.
Genomic regions Chromosome 8
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LOXL2-201 ENST00000389131.8 3721 774aa Protein coding CCDS34864 Q9Y4K0 NM_002318.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LOXL2-214 ENST00000524144.5 975 258aa Protein coding - E5RFY0 - CDS 3' incomplete, TSL:5,
LOXL2-209 ENST00000520871.1 892 218aa Protein coding - E5RHH3 - CDS 3' incomplete, TSL:2,
LOXL2-205 ENST00000519243.1 763 95aa Protein coding - E5RFE2 - CDS 3' incomplete, TSL:2,
LOXL2-215 ENST00000524168.1 746 139aa Protein coding - E5RI22 - CDS 3' incomplete, TSL:3,
LOXL2-207 ENST00000520349.1 620 207aa Protein coding - H0YAR1 - CDS 5' and 3' incomplete, TSL:3,
LOXL2-202 ENST00000518083.5 588 151aa Protein coding - E5RJL2 - CDS 3' incomplete, TSL:4,
LOXL2-212 ENST00000523833.2 559 101aa Protein coding - R4GMS2 - CDS 3' incomplete, TSL:5,
LOXL2-204 ENST00000518878.5 689 67aa Nonsense mediated decay - H0YAP6 - CDS 5' incomplete, TSL:3,
LOXL2-203 ENST00000518472.1 725 No protein Processed transcript - - - TSL:3,
LOXL2-206 ENST00000519809.5 571 No protein Processed transcript - - - TSL:4,
LOXL2-210 ENST00000520925.1 965 No protein Retained intron - - - TSL:2,
LOXL2-211 ENST00000522446.5 665 No protein Retained intron - - - TSL:3,
LOXL2-208 ENST00000520617.1 502 No protein Retained intron - - - TSL:2,
LOXL2-213 ENST00000524075.1 419 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LOXL2
This KO Strategy loading
Red Cotton™ Notes Gene LOXL2 had been KO in mcf7 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×