CRISPR-U™ Gene Knockout Cell Line Strategy

BRD2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human BRD2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol BRD2
Gene id 6046
Organism Homo sapiens
Official full symbol bromodomain containing 2
Gene type protein-coding
Also known as BRD2-IT1, D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3
Summary This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.
Genomic regions Chromosome 6
Strategy Summary
This gene has 10 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
BRD2-201 ENST00000374825.9 4956 801aa Protein coding CCDS4762 P25440-1 NM_005104.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
BRD2-202 ENST00000374831.8 4807 801aa Protein coding CCDS4762 P25440-1 - TSL:1, GENCODE basic, APPRIS P2,
BRD2-222 ENST00000678250.1 4798 801aa Protein coding CCDS4762 A0A024RCR5 - GENCODE basic, APPRIS P2,
BRD2-205 ENST00000449085.4 4579 801aa Protein coding CCDS4762 P25440-3 - TSL:1, GENCODE basic, APPRIS P2,
BRD2-203 ENST00000395287.5 3467 836aa Protein coding CCDS56420 P25440-2 - TSL:1, GENCODE basic, APPRIS ALT2,
BRD2-204 ENST00000449025.5 3210 807aa Protein coding - H0Y6K2 - CDS 5' incomplete, TSL:1,
BRD2-219 ENST00000607833.5 2338 552aa Protein coding - U3KQA6 - CDS 3' incomplete, TSL:1,
BRD2-206 ENST00000456339.5 887 296aa Protein coding - H0Y799 - CDS 5' and 3' incomplete, TSL:4,
BRD2-214 ENST00000496118.2 735 162aa Protein coding - J3QR72 - TSL:1, GENCODE basic,
BRD2-218 ENST00000606059.1 565 159aa Protein coding - U3KPW0 - CDS 5' incomplete, TSL:2,
BRD2-212 ENST00000482914.5 4834 147aa Nonsense mediated decay - E9PKQ9 - TSL:1,
BRD2-220 ENST00000677085.1 4312 147aa Nonsense mediated decay - - - -
BRD2-225 ENST00000679179.1 4282 147aa Nonsense mediated decay - - - -
BRD2-223 ENST00000678778.1 4064 401aa Nonsense mediated decay - - - -
BRD2-213 ENST00000495733.5 3404 401aa Nonsense mediated decay - E9PIQ3 - TSL:2,
BRD2-210 ENST00000481259.1 667 137aa Nonsense mediated decay - H0YDJ7 - CDS 5' incomplete, TSL:3,
BRD2-207 ENST00000463639.2 3045 No protein Processed transcript - - - TSL:2,
BRD2-215 ENST00000580234.1 718 No protein Processed transcript - - - TSL:4,
BRD2-216 ENST00000581002.1 550 No protein Processed transcript - - - TSL:4,
BRD2-217 ENST00000584808.1 547 No protein Processed transcript - - - TSL:4,
BRD2-209 ENST00000469132.2 6084 No protein Retained intron - - - TSL:2,
BRD2-224 ENST00000679099.1 4737 No protein Retained intron - - - -
BRD2-208 ENST00000464592.6 4520 No protein Retained intron - - - TSL:5,
BRD2-221 ENST00000677331.1 4144 No protein Retained intron - - - -
BRD2-211 ENST00000482838.1 759 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene BRD2
This KO Strategy loading
Red Cotton™ Notes Gene BRD2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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