CRISPR-U™ Gene Knockout Cell Line
Strategy
BRD2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human BRD2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | BRD2 |
| Gene id | 6046 |
| Organism | Homo sapiens |
| Official full symbol | bromodomain containing 2 |
| Gene type | protein-coding |
| Also known as | BRD2-IT1, D6S113E, FSH, FSRG1, NAT, O27.1.1, RING3, RNF3 |
| Summary | This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 10 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| BRD2-201 | ENST00000374825.9 | 4956 | 801aa | Protein coding | CCDS4762 | P25440-1 | NM_005104.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| BRD2-202 | ENST00000374831.8 | 4807 | 801aa | Protein coding | CCDS4762 | P25440-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| BRD2-222 | ENST00000678250.1 | 4798 | 801aa | Protein coding | CCDS4762 | A0A024RCR5 | - | GENCODE basic, APPRIS P2, |
| BRD2-205 | ENST00000449085.4 | 4579 | 801aa | Protein coding | CCDS4762 | P25440-3 | - | TSL:1, GENCODE basic, APPRIS P2, |
| BRD2-203 | ENST00000395287.5 | 3467 | 836aa | Protein coding | CCDS56420 | P25440-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| BRD2-204 | ENST00000449025.5 | 3210 | 807aa | Protein coding | - | H0Y6K2 | - | CDS 5' incomplete, TSL:1, |
| BRD2-219 | ENST00000607833.5 | 2338 | 552aa | Protein coding | - | U3KQA6 | - | CDS 3' incomplete, TSL:1, |
| BRD2-206 | ENST00000456339.5 | 887 | 296aa | Protein coding | - | H0Y799 | - | CDS 5' and 3' incomplete, TSL:4, |
| BRD2-214 | ENST00000496118.2 | 735 | 162aa | Protein coding | - | J3QR72 | - | TSL:1, GENCODE basic, |
| BRD2-218 | ENST00000606059.1 | 565 | 159aa | Protein coding | - | U3KPW0 | - | CDS 5' incomplete, TSL:2, |
| BRD2-212 | ENST00000482914.5 | 4834 | 147aa | Nonsense mediated decay | - | E9PKQ9 | - | TSL:1, |
| BRD2-220 | ENST00000677085.1 | 4312 | 147aa | Nonsense mediated decay | - | - | - | - |
| BRD2-225 | ENST00000679179.1 | 4282 | 147aa | Nonsense mediated decay | - | - | - | - |
| BRD2-223 | ENST00000678778.1 | 4064 | 401aa | Nonsense mediated decay | - | - | - | - |
| BRD2-213 | ENST00000495733.5 | 3404 | 401aa | Nonsense mediated decay | - | E9PIQ3 | - | TSL:2, |
| BRD2-210 | ENST00000481259.1 | 667 | 137aa | Nonsense mediated decay | - | H0YDJ7 | - | CDS 5' incomplete, TSL:3, |
| BRD2-207 | ENST00000463639.2 | 3045 | No protein | Processed transcript | - | - | - | TSL:2, |
| BRD2-215 | ENST00000580234.1 | 718 | No protein | Processed transcript | - | - | - | TSL:4, |
| BRD2-216 | ENST00000581002.1 | 550 | No protein | Processed transcript | - | - | - | TSL:4, |
| BRD2-217 | ENST00000584808.1 | 547 | No protein | Processed transcript | - | - | - | TSL:4, |
| BRD2-209 | ENST00000469132.2 | 6084 | No protein | Retained intron | - | - | - | TSL:2, |
| BRD2-224 | ENST00000679099.1 | 4737 | No protein | Retained intron | - | - | - | - |
| BRD2-208 | ENST00000464592.6 | 4520 | No protein | Retained intron | - | - | - | TSL:5, |
| BRD2-221 | ENST00000677331.1 | 4144 | No protein | Retained intron | - | - | - | - |
| BRD2-211 | ENST00000482838.1 | 759 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | BRD2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene BRD2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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