CRISPR-U™ Gene Knockout Cell Line Strategy

HLA-E Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HLA-E Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HLA-E
Gene id 3133
Organism Homo sapiens
Official full symbol major histocompatibility complex, class I, E
Gene type protein-coding
Also known as HLA-6.2, QA1
Summary HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
Genomic regions Chromosome 6
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HLA-E-201 ENST00000376630.5 2548 358aa Protein coding CCDS34379 A0A4E9D3W4 P13747 NM_005516.6 TSL:NA, GENCODE basic, APPRIS P1, MANE Select v0.92,
HLA-E-203 ENST00000493699.1 907 No protein Retained intron - - - TSL:NA,
HLA-E-202 ENST00000484194.1 726 No protein Retained intron - - - TSL:NA,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HLA-E
This KO Strategy loading
Red Cotton™ Notes Gene HLA-E had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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