CRISPR-U™ Gene Knockout Cell Line
Strategy
HLA-E Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HLA-E Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HLA-E |
| Gene id | 3133 |
| Organism | Homo sapiens |
| Official full symbol | major histocompatibility complex, class I, E |
| Gene type | protein-coding |
| Also known as | HLA-6.2, QA1 |
| Summary | HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HLA-E-201 | ENST00000376630.5 | 2548 | 358aa | Protein coding | CCDS34379 | A0A4E9D3W4 P13747 | NM_005516.6 | TSL:NA, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HLA-E-203 | ENST00000493699.1 | 907 | No protein | Retained intron | - | - | - | TSL:NA, |
| HLA-E-202 | ENST00000484194.1 | 726 | No protein | Retained intron | - | - | - | TSL:NA, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HLA-E |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HLA-E had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: