CRISPR-U™ Gene Knockout Cell Line
Strategy
ABCD3 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ABCD3 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ABCD3 |
| Gene id | 5825 |
| Organism | Homo sapiens |
| Official full symbol | ATP binding cassette subfamily D member 3 |
| Gene type | protein-coding |
| Also known as | ABC43, CBAS5, PMP70, PXMP1, ZWS2 |
| Summary | The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms. |
| Genomic regions | Chromosome 1 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ABCD3-202 | ENST00000370214.9 | 3604 | 659aa | Protein coding | CCDS749 | P28288-1 | NM_002858.4 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| ABCD3-201 | ENST00000315713.5 | 884 | 236aa | Protein coding | CCDS44175 | P28288-3 | - | TSL:1, GENCODE basic, |
| ABCD3-207 | ENST00000647998.2 | 3538 | 659aa | Protein coding | - | A0A3B3ITW3 | - | APPRIS ALT1, |
| ABCD3-205 | ENST00000484213.1 | 4367 | No protein | Processed transcript | - | - | - | TSL:1, |
| ABCD3-203 | ENST00000464165.1 | 3174 | No protein | Processed transcript | - | - | - | TSL:2, |
| ABCD3-204 | ENST00000468860.1 | 661 | No protein | Processed transcript | - | - | - | TSL:3, |
| ABCD3-206 | ENST00000493416.5 | 623 | No protein | Processed transcript | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ABCD3 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ABCD3 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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