CRISPR-U™ Gene Knockout Cell Line Strategy

ABCD3 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ABCD3 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ABCD3
Gene id 5825
Organism Homo sapiens
Official full symbol ATP binding cassette subfamily D member 3
Gene type protein-coding
Also known as ABC43, CBAS5, PMP70, PXMP1, ZWS2
Summary The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Genomic regions Chromosome 1
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ABCD3-202 ENST00000370214.9 3604 659aa Protein coding CCDS749 P28288-1 NM_002858.4 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
ABCD3-201 ENST00000315713.5 884 236aa Protein coding CCDS44175 P28288-3 - TSL:1, GENCODE basic,
ABCD3-207 ENST00000647998.2 3538 659aa Protein coding - A0A3B3ITW3 - APPRIS ALT1,
ABCD3-205 ENST00000484213.1 4367 No protein Processed transcript - - - TSL:1,
ABCD3-203 ENST00000464165.1 3174 No protein Processed transcript - - - TSL:2,
ABCD3-204 ENST00000468860.1 661 No protein Processed transcript - - - TSL:3,
ABCD3-206 ENST00000493416.5 623 No protein Processed transcript - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ABCD3
This KO Strategy loading
Red Cotton™ Notes Gene ABCD3 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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