CRISPR-U™ Gene Knockout Cell Line Strategy

B2M Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human B2M Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol B2M
Gene id 567
Organism Homo sapiens
Official full symbol beta-2-microglobulin
Gene type protein-coding
Also known as IMD43
Summary This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
Genomic regions Chromosome 15
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
B2M-207 ENST00000559916.1 1081 119aa Protein coding CCDS10113 P61769 - TSL:1, GENCODE basic, APPRIS P1,
B2M-214 ENST00000648006.3 943 119aa Protein coding CCDS10113 P61769 NM_004048.4 GENCODE basic, APPRIS P1, MANE Select v0.92,
B2M-202 ENST00000544417.5 933 101aa Protein coding - F5H6I0 - TSL:5, GENCODE basic,
B2M-211 ENST00000561424.5 604 119aa Nonsense mediated decay CCDS10113 P61769 - TSL:3,
B2M-201 ENST00000349264.10 732 57aa Nonsense mediated decay - J3KNU0 - TSL:3,
B2M-210 ENST00000561139.1 717 71aa Nonsense mediated decay - H0YLF3 - CDS 5' incomplete, TSL:3,
B2M-203 ENST00000557901.5 407 51aa Nonsense mediated decay - Q16446 - TSL:1,
B2M-204 ENST00000559220.1 580 No protein Processed transcript - - - TSL:5,
B2M-206 ENST00000559907.5 499 No protein Processed transcript - - - TSL:3,
B2M-208 ENST00000560556.1 472 No protein Processed transcript - - - TSL:3,
B2M-213 ENST00000632133.1 2403 No protein Retained intron - - - TSL:NA,
B2M-212 ENST00000623550.1 2329 No protein Retained intron - - - TSL:NA,
B2M-205 ENST00000559720.5 1220 No protein Retained intron - - - TSL:5,
B2M-209 ENST00000560681.1 572 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene B2M
This KO Strategy loading
Red Cotton™ Notes Gene B2M had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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