CRISPR-U™ Gene Knockout Cell Line
Strategy
B2M Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human B2M Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | B2M |
| Gene id | 567 |
| Organism | Homo sapiens |
| Official full symbol | beta-2-microglobulin |
| Gene type | protein-coding |
| Also known as | IMD43 |
| Summary | This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia. |
| Genomic regions | Chromosome 15 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| B2M-207 | ENST00000559916.1 | 1081 | 119aa | Protein coding | CCDS10113 | P61769 | - | TSL:1, GENCODE basic, APPRIS P1, |
| B2M-214 | ENST00000648006.3 | 943 | 119aa | Protein coding | CCDS10113 | P61769 | NM_004048.4 | GENCODE basic, APPRIS P1, MANE Select v0.92, |
| B2M-202 | ENST00000544417.5 | 933 | 101aa | Protein coding | - | F5H6I0 | - | TSL:5, GENCODE basic, |
| B2M-211 | ENST00000561424.5 | 604 | 119aa | Nonsense mediated decay | CCDS10113 | P61769 | - | TSL:3, |
| B2M-201 | ENST00000349264.10 | 732 | 57aa | Nonsense mediated decay | - | J3KNU0 | - | TSL:3, |
| B2M-210 | ENST00000561139.1 | 717 | 71aa | Nonsense mediated decay | - | H0YLF3 | - | CDS 5' incomplete, TSL:3, |
| B2M-203 | ENST00000557901.5 | 407 | 51aa | Nonsense mediated decay | - | Q16446 | - | TSL:1, |
| B2M-204 | ENST00000559220.1 | 580 | No protein | Processed transcript | - | - | - | TSL:5, |
| B2M-206 | ENST00000559907.5 | 499 | No protein | Processed transcript | - | - | - | TSL:3, |
| B2M-208 | ENST00000560556.1 | 472 | No protein | Processed transcript | - | - | - | TSL:3, |
| B2M-213 | ENST00000632133.1 | 2403 | No protein | Retained intron | - | - | - | TSL:NA, |
| B2M-212 | ENST00000623550.1 | 2329 | No protein | Retained intron | - | - | - | TSL:NA, |
| B2M-205 | ENST00000559720.5 | 1220 | No protein | Retained intron | - | - | - | TSL:5, |
| B2M-209 | ENST00000560681.1 | 572 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | B2M |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene B2M had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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