CRISPR-U™ Gene Knockout Cell Line Strategy

MTAP Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MTAP Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MTAP
Gene id 4507
Organism Homo sapiens
Official full symbol methylthioadenosine phosphorylase
Gene type protein-coding
Also known as BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus
Summary This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.
Genomic regions Chromosome 9
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MTAP-213 ENST00000644715.2 6032 283aa Protein coding CCDS6509 Q13126-1 NM_002451.4 GENCODE basic, APPRIS P2, MANE Select v0.92,
MTAP-209 ENST00000580900.5 7557 334aa Protein coding - Q13126-3 - TSL:1, GENCODE basic,
MTAP-203 ENST00000460874.6 1434 300aa Protein coding - B4DUC8 - TSL:2, GENCODE basic, APPRIS ALT2,
MTAP-202 ENST00000427788.2 753 55aa Protein coding - A0A2R8Y4K0 - CDS 3' incomplete, TSL:5,
MTAP-205 ENST00000577563.1 339 112aa Protein coding - J3KRN1 - CDS 5' incomplete, TSL:1,
MTAP-201 ENST00000419385.5 2241 50aa Nonsense mediated decay - F8WES2 - TSL:5,
MTAP-208 ENST00000580718.1 1309 242aa Nonsense mediated decay - J3QSB7 - TSL:1,
MTAP-211 ENST00000584988.1 611 No protein Processed transcript - - - TSL:3,
MTAP-206 ENST00000579422.5 578 No protein Processed transcript - - - TSL:5,
MTAP-204 ENST00000484957.1 399 No protein Processed transcript - - - TSL:3,
MTAP-212 ENST00000616982.1 2350 No protein Retained intron - - - TSL:NA,
MTAP-210 ENST00000581962.1 323 No protein Retained intron - - - TSL:2,
MTAP-207 ENST00000580675.1 310 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MTAP
This KO Strategy loading
Red Cotton™ Notes Gene MTAP had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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