CRISPR-U™ Gene Knockout Cell Line
Strategy
MTAP Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MTAP Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MTAP |
| Gene id | 4507 |
| Organism | Homo sapiens |
| Official full symbol | methylthioadenosine phosphorylase |
| Gene type | protein-coding |
| Also known as | BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus |
| Summary | This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MTAP-213 | ENST00000644715.2 | 6032 | 283aa | Protein coding | CCDS6509 | Q13126-1 | NM_002451.4 | GENCODE basic, APPRIS P2, MANE Select v0.92, |
| MTAP-209 | ENST00000580900.5 | 7557 | 334aa | Protein coding | - | Q13126-3 | - | TSL:1, GENCODE basic, |
| MTAP-203 | ENST00000460874.6 | 1434 | 300aa | Protein coding | - | B4DUC8 | - | TSL:2, GENCODE basic, APPRIS ALT2, |
| MTAP-202 | ENST00000427788.2 | 753 | 55aa | Protein coding | - | A0A2R8Y4K0 | - | CDS 3' incomplete, TSL:5, |
| MTAP-205 | ENST00000577563.1 | 339 | 112aa | Protein coding | - | J3KRN1 | - | CDS 5' incomplete, TSL:1, |
| MTAP-201 | ENST00000419385.5 | 2241 | 50aa | Nonsense mediated decay | - | F8WES2 | - | TSL:5, |
| MTAP-208 | ENST00000580718.1 | 1309 | 242aa | Nonsense mediated decay | - | J3QSB7 | - | TSL:1, |
| MTAP-211 | ENST00000584988.1 | 611 | No protein | Processed transcript | - | - | - | TSL:3, |
| MTAP-206 | ENST00000579422.5 | 578 | No protein | Processed transcript | - | - | - | TSL:5, |
| MTAP-204 | ENST00000484957.1 | 399 | No protein | Processed transcript | - | - | - | TSL:3, |
| MTAP-212 | ENST00000616982.1 | 2350 | No protein | Retained intron | - | - | - | TSL:NA, |
| MTAP-210 | ENST00000581962.1 | 323 | No protein | Retained intron | - | - | - | TSL:2, |
| MTAP-207 | ENST00000580675.1 | 310 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MTAP |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MTAP had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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