CRISPR-U™ Gene Knockout Cell Line Strategy

CASP8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CASP8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CASP8
Gene id 841
Organism Homo sapiens
Official full symbol caspase 8
Gene type protein-coding
Also known as ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5
Summary This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined.
Genomic regions Chromosome 2
Strategy Summary
This gene has 16 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CASP8-205 ENST00000358485.8 2930 538aa Protein coding CCDS42798 Q14790-9 - TSL:1, GENCODE basic, APPRIS P4,
CASP8-221 ENST00000673742.1 2659 479aa Protein coding CCDS2342 Q14790-1 NM_001372051.1 GENCODE basic, APPRIS ALT2, MANE Select v0.92,
CASP8-203 ENST00000323492.11 2650 464aa Protein coding CCDS2343 Q14790-2 - TSL:1, GENCODE basic, APPRIS ALT2,
CASP8-202 ENST00000264275.9 1906 496aa Protein coding CCDS42799 Q14790-4 - TSL:1, GENCODE basic,
CASP8-207 ENST00000392263.6 1630 464aa Protein coding CCDS2343 Q14790-2 - CDS 3' incomplete, TSL:5,
CASP8-212 ENST00000432109.6 1629 479aa Protein coding CCDS2342 Q14790-1 - TSL:5, GENCODE basic, APPRIS ALT2,
CASP8-206 ENST00000392258.7 1060 235aa Protein coding CCDS2345 Q14790-5 - TSL:1, GENCODE basic,
CASP8-201 ENST00000264274.13 2540 395aa Protein coding - Q14790-3 - TSL:5, GENCODE basic,
CASP8-208 ENST00000392266.7 1283 278aa Protein coding - A0A0A0MS31 - TSL:2, GENCODE basic,
CASP8-215 ENST00000444430.2 1275 258aa Protein coding - H7C0E2 - CDS 5' incomplete, TSL:5,
CASP8-211 ENST00000429881.1 717 116aa Protein coding - E7EQ01 - CDS 3' incomplete, TSL:3,
CASP8-216 ENST00000447616.5 707 209aa Protein coding - E7EQ06 - CDS 3' incomplete, TSL:5,
CASP8-217 ENST00000450491.5 686 149aa Protein coding - C9JB29 - CDS 3' incomplete, TSL:3,
CASP8-209 ENST00000413726.5 658 198aa Protein coding - E7ETB7 - CDS 3' incomplete, TSL:5,
CASP8-214 ENST00000440732.5 592 124aa Protein coding - E7EVN1 - CDS 3' incomplete, TSL:4,
CASP8-210 ENST00000424461.5 308 83aa Protein coding - H7BZM4 - CDS 5' incomplete, TSL:5,
CASP8-213 ENST00000437283.5 777 58aa Nonsense mediated decay - F8WF39 - TSL:1,
CASP8-220 ENST00000490682.5 2911 No protein Processed transcript - - - TSL:1,
CASP8-219 ENST00000490412.5 230 No protein Processed transcript - - - TSL:1,
CASP8-204 ENST00000339403.6 1723 No protein Retained intron - - - TSL:1,
CASP8-218 ENST00000471383.5 969 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CASP8
This KO Strategy loading
Red Cotton™ Notes Gene CASP8 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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