CRISPR-U™ Gene Knockout Cell Line
Strategy
CASP8 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CASP8 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CASP8 |
| Gene id | 841 |
| Organism | Homo sapiens |
| Official full symbol | caspase 8 |
| Gene type | protein-coding |
| Also known as | ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 |
| Summary | This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 16 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CASP8-205 | ENST00000358485.8 | 2930 | 538aa | Protein coding | CCDS42798 | Q14790-9 | - | TSL:1, GENCODE basic, APPRIS P4, |
| CASP8-221 | ENST00000673742.1 | 2659 | 479aa | Protein coding | CCDS2342 | Q14790-1 | NM_001372051.1 | GENCODE basic, APPRIS ALT2, MANE Select v0.92, |
| CASP8-203 | ENST00000323492.11 | 2650 | 464aa | Protein coding | CCDS2343 | Q14790-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| CASP8-202 | ENST00000264275.9 | 1906 | 496aa | Protein coding | CCDS42799 | Q14790-4 | - | TSL:1, GENCODE basic, |
| CASP8-207 | ENST00000392263.6 | 1630 | 464aa | Protein coding | CCDS2343 | Q14790-2 | - | CDS 3' incomplete, TSL:5, |
| CASP8-212 | ENST00000432109.6 | 1629 | 479aa | Protein coding | CCDS2342 | Q14790-1 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| CASP8-206 | ENST00000392258.7 | 1060 | 235aa | Protein coding | CCDS2345 | Q14790-5 | - | TSL:1, GENCODE basic, |
| CASP8-201 | ENST00000264274.13 | 2540 | 395aa | Protein coding | - | Q14790-3 | - | TSL:5, GENCODE basic, |
| CASP8-208 | ENST00000392266.7 | 1283 | 278aa | Protein coding | - | A0A0A0MS31 | - | TSL:2, GENCODE basic, |
| CASP8-215 | ENST00000444430.2 | 1275 | 258aa | Protein coding | - | H7C0E2 | - | CDS 5' incomplete, TSL:5, |
| CASP8-211 | ENST00000429881.1 | 717 | 116aa | Protein coding | - | E7EQ01 | - | CDS 3' incomplete, TSL:3, |
| CASP8-216 | ENST00000447616.5 | 707 | 209aa | Protein coding | - | E7EQ06 | - | CDS 3' incomplete, TSL:5, |
| CASP8-217 | ENST00000450491.5 | 686 | 149aa | Protein coding | - | C9JB29 | - | CDS 3' incomplete, TSL:3, |
| CASP8-209 | ENST00000413726.5 | 658 | 198aa | Protein coding | - | E7ETB7 | - | CDS 3' incomplete, TSL:5, |
| CASP8-214 | ENST00000440732.5 | 592 | 124aa | Protein coding | - | E7EVN1 | - | CDS 3' incomplete, TSL:4, |
| CASP8-210 | ENST00000424461.5 | 308 | 83aa | Protein coding | - | H7BZM4 | - | CDS 5' incomplete, TSL:5, |
| CASP8-213 | ENST00000437283.5 | 777 | 58aa | Nonsense mediated decay | - | F8WF39 | - | TSL:1, |
| CASP8-220 | ENST00000490682.5 | 2911 | No protein | Processed transcript | - | - | - | TSL:1, |
| CASP8-219 | ENST00000490412.5 | 230 | No protein | Processed transcript | - | - | - | TSL:1, |
| CASP8-204 | ENST00000339403.6 | 1723 | No protein | Retained intron | - | - | - | TSL:1, |
| CASP8-218 | ENST00000471383.5 | 969 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CASP8 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CASP8 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: