CRISPR-U™ Gene Knockout Cell Line Strategy

COMT Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COMT Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol COMT
Gene id 1312
Organism Homo sapiens
Official full symbol catechol-O-methyltransferase
Gene type protein-coding
Also known as HEL-S-98n
Summary Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
Genomic regions Chromosome 22
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
COMT-218 ENST00000678255.1 3119 271aa Protein coding CCDS13770 A0A140VJG8 - GENCODE basic, APPRIS ALT2,
COMT-220 ENST00000678868.1 2405 271aa Protein coding CCDS13770 A0A140VJG8 - GENCODE basic, APPRIS ALT2,
COMT-202 ENST00000361682.11 2272 271aa Protein coding CCDS13770 P21964-1 NM_000754.4 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
COMT-204 ENST00000403710.5 1548 271aa Protein coding CCDS13770 P21964-1 - TSL:5, GENCODE basic, APPRIS P4,
COMT-206 ENST00000407537.5 1457 271aa Protein coding CCDS13770 P21964-1 - TSL:5, GENCODE basic, APPRIS P4,
COMT-212 ENST00000676678.1 1405 271aa Protein coding CCDS13770 A0A140VJG8 - GENCODE basic, APPRIS ALT2,
COMT-205 ENST00000406520.7 1339 271aa Protein coding CCDS13770 P21964-1 - TSL:1, GENCODE basic, APPRIS P4,
COMT-209 ENST00000449653.5 1035 221aa Protein coding CCDS46663 P21964-2 - TSL:1, GENCODE basic, APPRIS ALT2,
COMT-208 ENST00000428707.2 2492 292aa Protein coding - H7BZ45 - TSL:3, GENCODE basic,
COMT-203 ENST00000403184.5 2217 235aa Protein coding - E7EUU8 - TSL:2, GENCODE basic,
COMT-219 ENST00000678769.1 2202 302aa Protein coding - - - GENCODE basic, APPRIS ALT2,
COMT-207 ENST00000412786.5 871 223aa Protein coding - E7EMS6 - CDS 3' incomplete, TSL:2,
COMT-213 ENST00000677397.1 1363 242aa Nonsense mediated decay - - - -
COMT-201 ENST00000207636.9 1319 199aa Nonsense mediated decay - F8WBW9 - TSL:5,
COMT-216 ENST00000677675.1 909 No protein Processed transcript - - - -
COMT-211 ENST00000493893.1 354 No protein Processed transcript - - - TSL:3,
COMT-221 ENST00000678945.1 1903 No protein Retained intron - - - -
COMT-215 ENST00000677564.1 1818 No protein Retained intron - - - -
COMT-214 ENST00000677470.1 959 No protein Retained intron - - - -
COMT-217 ENST00000678240.1 617 No protein Retained intron - - - -
COMT-210 ENST00000467943.5 546 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene COMT
This KO Strategy loading
Red Cotton™ Notes Gene COMT had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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