CRISPR-U™ Gene Knockout Cell Line
Strategy
COMT Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human COMT Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | COMT |
| Gene id | 1312 |
| Organism | Homo sapiens |
| Official full symbol | catechol-O-methyltransferase |
| Gene type | protein-coding |
| Also known as | HEL-S-98n |
| Summary | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. |
| Genomic regions | Chromosome 22 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| COMT-218 | ENST00000678255.1 | 3119 | 271aa | Protein coding | CCDS13770 | A0A140VJG8 | - | GENCODE basic, APPRIS ALT2, |
| COMT-220 | ENST00000678868.1 | 2405 | 271aa | Protein coding | CCDS13770 | A0A140VJG8 | - | GENCODE basic, APPRIS ALT2, |
| COMT-202 | ENST00000361682.11 | 2272 | 271aa | Protein coding | CCDS13770 | P21964-1 | NM_000754.4 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| COMT-204 | ENST00000403710.5 | 1548 | 271aa | Protein coding | CCDS13770 | P21964-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| COMT-206 | ENST00000407537.5 | 1457 | 271aa | Protein coding | CCDS13770 | P21964-1 | - | TSL:5, GENCODE basic, APPRIS P4, |
| COMT-212 | ENST00000676678.1 | 1405 | 271aa | Protein coding | CCDS13770 | A0A140VJG8 | - | GENCODE basic, APPRIS ALT2, |
| COMT-205 | ENST00000406520.7 | 1339 | 271aa | Protein coding | CCDS13770 | P21964-1 | - | TSL:1, GENCODE basic, APPRIS P4, |
| COMT-209 | ENST00000449653.5 | 1035 | 221aa | Protein coding | CCDS46663 | P21964-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| COMT-208 | ENST00000428707.2 | 2492 | 292aa | Protein coding | - | H7BZ45 | - | TSL:3, GENCODE basic, |
| COMT-203 | ENST00000403184.5 | 2217 | 235aa | Protein coding | - | E7EUU8 | - | TSL:2, GENCODE basic, |
| COMT-219 | ENST00000678769.1 | 2202 | 302aa | Protein coding | - | - | - | GENCODE basic, APPRIS ALT2, |
| COMT-207 | ENST00000412786.5 | 871 | 223aa | Protein coding | - | E7EMS6 | - | CDS 3' incomplete, TSL:2, |
| COMT-213 | ENST00000677397.1 | 1363 | 242aa | Nonsense mediated decay | - | - | - | - |
| COMT-201 | ENST00000207636.9 | 1319 | 199aa | Nonsense mediated decay | - | F8WBW9 | - | TSL:5, |
| COMT-216 | ENST00000677675.1 | 909 | No protein | Processed transcript | - | - | - | - |
| COMT-211 | ENST00000493893.1 | 354 | No protein | Processed transcript | - | - | - | TSL:3, |
| COMT-221 | ENST00000678945.1 | 1903 | No protein | Retained intron | - | - | - | - |
| COMT-215 | ENST00000677564.1 | 1818 | No protein | Retained intron | - | - | - | - |
| COMT-214 | ENST00000677470.1 | 959 | No protein | Retained intron | - | - | - | - |
| COMT-217 | ENST00000678240.1 | 617 | No protein | Retained intron | - | - | - | - |
| COMT-210 | ENST00000467943.5 | 546 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | COMT |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene COMT had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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