CRISPR-U™ Gene Knockout Cell Line
Strategy
LRP5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LRP5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | LRP5 |
| Gene id | 4041 |
| Organism | Homo sapiens |
| Official full symbol | LDL receptor related protein 5 |
| Gene type | protein-coding |
| Also known as | BMND1, EVR1, EVR4, HBM, LR3, LRP-5, LRP-7, LRP7, OPPG, OPS, OPTA1, PCLD4, VBCH2 |
| Summary | This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| LRP5-201 | ENST00000294304.12 | 5177 | 1615aa | Protein coding | CCDS8181 | O75197 | NM_002335.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| LRP5-205 | ENST00000529702.1 | 586 | 172aa | Protein coding | - | H0YE98 | - | CDS 5' incomplete, TSL:3, |
| LRP5-206 | ENST00000529993.5 | 5103 | 471aa | Nonsense mediated decay | - | E9PHY1 | - | TSL:1, |
| LRP5-204 | ENST00000529481.1 | 551 | No protein | Processed transcript | - | - | - | TSL:3, |
| LRP5-202 | ENST00000528714.1 | 534 | No protein | Processed transcript | - | - | - | TSL:3, |
| LRP5-203 | ENST00000528890.1 | 464 | No protein | Retained intron | - | - | - | TSL:3, |
| LRP5-207 | ENST00000533695.1 | 267 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | LRP5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene LRP5 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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