CRISPR-U™ Gene Knockout Cell Line Strategy

LRP5 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human LRP5 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LRP5
Gene id 4041
Organism Homo sapiens
Official full symbol LDL receptor related protein 5
Gene type protein-coding
Also known as BMND1, EVR1, EVR4, HBM, LR3, LRP-5, LRP-7, LRP7, OPPG, OPS, OPTA1, PCLD4, VBCH2
Summary This gene encodes a transmembrane low-density lipoprotein receptor that binds and internalizes ligands in the process of receptor-mediated endocytosis. This protein also acts as a co-receptor with Frizzled protein family members for transducing signals by Wnt proteins and was originally cloned on the basis of its association with type 1 diabetes mellitus in humans. This protein plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in this gene. Mutations in this gene also cause familial exudative vitreoretinopathy. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 11
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LRP5-201 ENST00000294304.12 5177 1615aa Protein coding CCDS8181 O75197 NM_002335.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LRP5-205 ENST00000529702.1 586 172aa Protein coding - H0YE98 - CDS 5' incomplete, TSL:3,
LRP5-206 ENST00000529993.5 5103 471aa Nonsense mediated decay - E9PHY1 - TSL:1,
LRP5-204 ENST00000529481.1 551 No protein Processed transcript - - - TSL:3,
LRP5-202 ENST00000528714.1 534 No protein Processed transcript - - - TSL:3,
LRP5-203 ENST00000528890.1 464 No protein Retained intron - - - TSL:3,
LRP5-207 ENST00000533695.1 267 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LRP5
This KO Strategy loading
Red Cotton™ Notes Gene LRP5 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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