CRISPR-U™ Gene Knockout Cell Line Strategy

ASS1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ASS1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ASS1
Gene id 445
Organism Homo sapiens
Official full symbol argininosuccinate synthase 1
Gene type protein-coding
Also known as ASS, CTLN1
Summary The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
Genomic regions Chromosome 9
Strategy Summary
This gene has 5 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ASS1-204 ENST00000372394.5 1973 412aa Protein coding CCDS6933 P00966 Q5T6L4 - TSL:2, GENCODE basic, APPRIS P1,
ASS1-203 ENST00000372393.7 1548 412aa Protein coding CCDS6933 P00966 Q5T6L4 - TSL:5, GENCODE basic, APPRIS P1,
ASS1-201 ENST00000352480.10 1532 412aa Protein coding CCDS6933 P00966 Q5T6L4 NM_054012.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ASS1-205 ENST00000422569.5 807 198aa Protein coding - Q5T6L6 - CDS 3' incomplete, TSL:5,
ASS1-206 ENST00000443588.1 545 179aa Protein coding - Q5T6L5 - CDS 3' incomplete, TSL:5,
ASS1-202 ENST00000372386.6 667 No protein Processed transcript - - - TSL:3,
ASS1-209 ENST00000492400.5 444 No protein Processed transcript - - - TSL:3,
ASS1-210 ENST00000493984.6 687 No protein Retained intron - - - TSL:5,
ASS1-208 ENST00000470849.4 652 No protein Retained intron - - - TSL:2,
ASS1-207 ENST00000467695.5 580 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ASS1
This KO Strategy loading
Red Cotton™ Notes Gene ASS1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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