CRISPR-U™ Gene Knockout Cell Line
Strategy
ASS1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ASS1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ASS1 |
| Gene id | 445 |
| Organism | Homo sapiens |
| Official full symbol | argininosuccinate synthase 1 |
| Gene type | protein-coding |
| Also known as | ASS, CTLN1 |
| Summary | The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of this gene cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 5 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ASS1-204 | ENST00000372394.5 | 1973 | 412aa | Protein coding | CCDS6933 | P00966 Q5T6L4 | - | TSL:2, GENCODE basic, APPRIS P1, |
| ASS1-203 | ENST00000372393.7 | 1548 | 412aa | Protein coding | CCDS6933 | P00966 Q5T6L4 | - | TSL:5, GENCODE basic, APPRIS P1, |
| ASS1-201 | ENST00000352480.10 | 1532 | 412aa | Protein coding | CCDS6933 | P00966 Q5T6L4 | NM_054012.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ASS1-205 | ENST00000422569.5 | 807 | 198aa | Protein coding | - | Q5T6L6 | - | CDS 3' incomplete, TSL:5, |
| ASS1-206 | ENST00000443588.1 | 545 | 179aa | Protein coding | - | Q5T6L5 | - | CDS 3' incomplete, TSL:5, |
| ASS1-202 | ENST00000372386.6 | 667 | No protein | Processed transcript | - | - | - | TSL:3, |
| ASS1-209 | ENST00000492400.5 | 444 | No protein | Processed transcript | - | - | - | TSL:3, |
| ASS1-210 | ENST00000493984.6 | 687 | No protein | Retained intron | - | - | - | TSL:5, |
| ASS1-208 | ENST00000470849.4 | 652 | No protein | Retained intron | - | - | - | TSL:2, |
| ASS1-207 | ENST00000467695.5 | 580 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ASS1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ASS1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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