CRISPR-U™ Gene Knockout Cell Line
Strategy
DAXX Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DAXX Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DAXX |
| Gene id | 1616 |
| Organism | Homo sapiens |
| Official full symbol | death domain associated protein |
| Gene type | protein-coding |
| Also known as | BING2, DAP6, EAP1, SMIM40 |
| Summary | This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DAXX-201 | ENST00000266000.10 | 2606 | 740aa | Protein coding | CCDS4776 | Q9UER7-1 | - | TSL:1, GENCODE basic, APPRIS P2, |
| DAXX-202 | ENST00000374542.10 | 2558 | 740aa | Protein coding | CCDS4776 | Q9UER7-1 | NM_001141969.2 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| DAXX-203 | ENST00000414083.6 | 2355 | 665aa | Protein coding | CCDS59008 | Q9UER7-3 | - | TSL:2, GENCODE basic, |
| DAXX-211 | ENST00000620164.4 | 2462 | 688aa | Protein coding | - | Q9UER7-4 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| DAXX-204 | ENST00000446403.1 | 702 | 178aa | Protein coding | - | Q4VX54 | - | CDS 3' incomplete, TSL:3, |
| DAXX-205 | ENST00000446511.1 | 371 | 48aa | Protein coding | - | A2AB94 | - | CDS 3' incomplete, TSL:5, |
| DAXX-207 | ENST00000477162.1 | 847 | No protein | Processed transcript | - | - | - | TSL:5, |
| DAXX-206 | ENST00000468536.5 | 817 | No protein | Processed transcript | - | - | - | TSL:2, |
| DAXX-208 | ENST00000477370.1 | 756 | No protein | Processed transcript | - | - | - | TSL:3, |
| DAXX-209 | ENST00000490173.1 | 641 | No protein | Retained intron | - | - | - | TSL:1, |
| DAXX-210 | ENST00000498030.1 | 463 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DAXX |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DAXX had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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