CRISPR-U™ Gene Knockout Cell Line Strategy

DAXX Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DAXX Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DAXX
Gene id 1616
Organism Homo sapiens
Official full symbol death domain associated protein
Gene type protein-coding
Also known as BING2, DAP6, EAP1, SMIM40
Summary This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 6
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DAXX-201 ENST00000266000.10 2606 740aa Protein coding CCDS4776 Q9UER7-1 - TSL:1, GENCODE basic, APPRIS P2,
DAXX-202 ENST00000374542.10 2558 740aa Protein coding CCDS4776 Q9UER7-1 NM_001141969.2 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
DAXX-203 ENST00000414083.6 2355 665aa Protein coding CCDS59008 Q9UER7-3 - TSL:2, GENCODE basic,
DAXX-211 ENST00000620164.4 2462 688aa Protein coding - Q9UER7-4 - TSL:5, GENCODE basic, APPRIS ALT2,
DAXX-204 ENST00000446403.1 702 178aa Protein coding - Q4VX54 - CDS 3' incomplete, TSL:3,
DAXX-205 ENST00000446511.1 371 48aa Protein coding - A2AB94 - CDS 3' incomplete, TSL:5,
DAXX-207 ENST00000477162.1 847 No protein Processed transcript - - - TSL:5,
DAXX-206 ENST00000468536.5 817 No protein Processed transcript - - - TSL:2,
DAXX-208 ENST00000477370.1 756 No protein Processed transcript - - - TSL:3,
DAXX-209 ENST00000490173.1 641 No protein Retained intron - - - TSL:1,
DAXX-210 ENST00000498030.1 463 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DAXX
This KO Strategy loading
Red Cotton™ Notes Gene DAXX had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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