CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ACE2 Knockout
model in DLD-1 cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info
Official symbol
ACE2
Gene id
59272
Organism
Homo sapiens
Official full symbol
angiotensin I converting enzyme 2
Gene type
protein-coding
Also known as
ACEH
Summary
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the causative agent of coronavirus disease-2019 (COVID-19).
Genomic regions
Chromosome X
Cell line info
Cell name
DLD-1
Tissue
colon
Organism
Human
Morphology
epithelial
Culture properties
adherent
Disease
Dukes' type C, colorectal adenocarcinoma
Derivation
DLD-1 is one of two colorectal adenocarcinoma cell lines which were isolated by D.L. Dexter and associates during a period from 1977-1979A culture of unknown passage submitted to the ATCC in 1979 was found to be contaminated with Mycoplasma hyorhinis.
Complete growth medium
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.
To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
This medium is formulated for use with a 5% CO2 in air atmosphere.
ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
Subcultivation ratio
A subcultivation ratio of 1:3 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Strategy Summary
This gene has 8 protein coding transcripts:
Name
Transcript ID
bp
Protein
Biotype
CCDS
UniProt Match
RefSeq Match
Flags
ACE2-202
ENST00000427411.2
7587
805aa
Protein coding
CCDS14169
Q9BYF1-1
-
TSL:1, GENCODE basic, APPRIS P2,
ACE2-207
ENST00000678073.1
7487
805aa
Protein coding
CCDS14169
-
-
GENCODE basic, APPRIS P2,
ACE2-211
ENST00000680121.1
7413
805aa
Protein coding
CCDS14169
-
-
GENCODE basic, APPRIS P2,
ACE2-201
ENST00000252519.8
3339
805aa
Protein coding
CCDS14169
Q9BYF1-1
NM_001371415.1
TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
Ubigene has successfully modified
DLD-1
cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.