CRISPR-U™ Gene Knockout Cell Line
Strategy
DAG1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DAG1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DAG1 |
| Gene id | 1605 |
| Organism | Homo sapiens |
| Official full symbol | dystroglycan 1 |
| Gene type | protein-coding |
| Also known as | 156DAG, A3a, AGRNR, DAG, LGMDR16, MDDGA9, MDDGC7, MDDGC9 |
| Summary | This gene encodes dystroglycan, a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. The encoded preproprotein undergoes O- and N-glycosylation, and proteolytic processing to generate alpha and beta subunits. Certain mutations in this gene are known to cause distinct forms of muscular dystrophy. Alternative splicing results in multiple transcript variants, all encoding the same protein. |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 16 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DAG1-221 | ENST00000545947.5 | 5829 | 895aa | Protein coding | CCDS2799 | Q14118 | - | TSL:5, GENCODE basic, APPRIS P1, |
| DAG1-220 | ENST00000541308.5 | 5676 | 895aa | Protein coding | CCDS2799 | Q14118 | - | TSL:5, GENCODE basic, APPRIS P1, |
| DAG1-219 | ENST00000539901.5 | 5665 | 895aa | Protein coding | CCDS2799 | Q14118 | - | TSL:5, GENCODE basic, APPRIS P1, |
| DAG1-218 | ENST00000538711.5 | 5582 | 895aa | Protein coding | CCDS2799 | Q14118 | - | TSL:5, GENCODE basic, APPRIS P1, |
| DAG1-217 | ENST00000515359.6 | 5576 | 895aa | Protein coding | CCDS2799 | Q14118 | - | TSL:5, GENCODE basic, APPRIS P1, |
| DAG1-201 | ENST00000308775.7 | 5387 | 895aa | Protein coding | CCDS2799 | Q14118 | NM_004393.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DAG1-222 | ENST00000673708.1 | 4517 | 895aa | Protein coding | CCDS2799 | Q14118 | - | GENCODE basic, APPRIS P1, |
| DAG1-204 | ENST00000428779.6 | 909 | 118aa | Protein coding | - | C9JYS1 | - | CDS 3' incomplete, TSL:3, |
| DAG1-202 | ENST00000418588.5 | 731 | 88aa | Protein coding | - | C9J6Z6 | - | CDS 3' incomplete, TSL:3, |
| DAG1-208 | ENST00000452060.6 | 730 | 134aa | Protein coding | - | C9JEN1 | - | CDS 3' incomplete, TSL:2, |
| DAG1-207 | ENST00000435508.7 | 624 | 77aa | Protein coding | - | C9JEH2 | - | CDS 3' incomplete, TSL:3, |
| DAG1-203 | ENST00000421560.5 | 579 | 106aa | Protein coding | - | C9J196 | - | CDS 3' incomplete, TSL:4, |
| DAG1-213 | ENST00000469139.1 | 561 | 34aa | Protein coding | - | A0A669KB80 | - | CDS 3' incomplete, TSL:4, |
| DAG1-206 | ENST00000431960.5 | 549 | 117aa | Protein coding | - | C9JQL4 | - | CDS 3' incomplete, TSL:4, |
| DAG1-209 | ENST00000452317.5 | 547 | 41aa | Protein coding | - | C9JY76 | - | CDS 3' incomplete, TSL:4, |
| DAG1-205 | ENST00000430636.1 | 397 | 8aa | Protein coding | - | A0A1D5RMP6 | - | CDS 3' incomplete, TSL:4, |
| DAG1-210 | ENST00000461492.1 | 580 | No protein | Processed transcript | - | - | - | TSL:3, |
| DAG1-216 | ENST00000496474.1 | 539 | No protein | Processed transcript | - | - | - | TSL:4, |
| DAG1-211 | ENST00000461987.2 | 386 | No protein | Processed transcript | - | - | - | TSL:3, |
| DAG1-212 | ENST00000466701.1 | 364 | No protein | Processed transcript | - | - | - | TSL:4, |
| DAG1-215 | ENST00000479935.1 | 711 | No protein | Retained intron | - | - | - | TSL:1, |
| DAG1-214 | ENST00000475424.1 | 581 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DAG1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DAG1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: