CRISPR-U™ Gene Knockout Cell Line
Strategy
NEK6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human NEK6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | NEK6 |
| Gene id | 10783 |
| Organism | Homo sapiens |
| Official full symbol | NIMA related kinase 6 |
| Gene type | protein-coding |
| Also known as | SID6-1512 |
| Summary | The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| NEK6-203 | ENST00000373600.7 | 3619 | 347aa | Protein coding | CCDS48015 | Q9HC98-2 | - | TSL:1, GENCODE basic, |
| NEK6-201 | ENST00000320246.10 | 3468 | 313aa | Protein coding | CCDS6854 | Q9HC98-1 | NM_014397.6 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| NEK6-204 | ENST00000373603.5 | 3447 | 313aa | Protein coding | CCDS6854 | Q9HC98-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NEK6-212 | ENST00000539416.5 | 2582 | 338aa | Protein coding | CCDS55339 | Q9HC98-4 | - | TSL:2, GENCODE basic, |
| NEK6-215 | ENST00000546191.5 | 2580 | 313aa | Protein coding | CCDS6854 | Q9HC98-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| NEK6-213 | ENST00000540326.5 | 2578 | 331aa | Protein coding | CCDS55338 | Q9HC98-3 | - | TSL:1, GENCODE basic, |
| NEK6-214 | ENST00000545174.5 | 2573 | 313aa | Protein coding | CCDS6854 | Q9HC98-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| NEK6-205 | ENST00000394199.6 | 2535 | 347aa | Protein coding | CCDS48015 | Q9HC98-2 | - | TSL:2, GENCODE basic, |
| NEK6-208 | ENST00000425237.5 | 806 | 222aa | Protein coding | - | Q5TBH1 | - | CDS 3' incomplete, TSL:3, |
| NEK6-206 | ENST00000422297.5 | 799 | 220aa | Protein coding | - | Q5TBH2 | - | CDS 3' incomplete, TSL:3, |
| NEK6-209 | ENST00000444973.5 | 790 | 219aa | Protein coding | - | Q5TBG7 | - | CDS 3' incomplete, TSL:5, |
| NEK6-202 | ENST00000373596.5 | 758 | 207aa | Protein coding | - | Q5TBG1 | - | CDS 3' incomplete, TSL:3, |
| NEK6-211 | ENST00000454453.5 | 732 | 200aa | Protein coding | - | Q5TBH0 | - | CDS 3' incomplete, TSL:3, |
| NEK6-207 | ENST00000423785.5 | 726 | 194aa | Protein coding | - | Q5TBG4 | - | CDS 3' incomplete, TSL:5, |
| NEK6-210 | ENST00000447379.1 | 716 | 171aa | Protein coding | - | Q5TBG2 | - | CDS 3' incomplete, TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | NEK6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene NEK6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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