CRISPR-U™ Gene Knockout Cell Line Strategy

NEK6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human NEK6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol NEK6
Gene id 10783
Organism Homo sapiens
Official full symbol NIMA related kinase 6
Gene type protein-coding
Also known as SID6-1512
Summary The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene.
Genomic regions Chromosome 9
Strategy Summary
This gene has 15 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
NEK6-203 ENST00000373600.7 3619 347aa Protein coding CCDS48015 Q9HC98-2 - TSL:1, GENCODE basic,
NEK6-201 ENST00000320246.10 3468 313aa Protein coding CCDS6854 Q9HC98-1 NM_014397.6 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
NEK6-204 ENST00000373603.5 3447 313aa Protein coding CCDS6854 Q9HC98-1 - TSL:1, GENCODE basic, APPRIS P1,
NEK6-212 ENST00000539416.5 2582 338aa Protein coding CCDS55339 Q9HC98-4 - TSL:2, GENCODE basic,
NEK6-215 ENST00000546191.5 2580 313aa Protein coding CCDS6854 Q9HC98-1 - TSL:5, GENCODE basic, APPRIS P1,
NEK6-213 ENST00000540326.5 2578 331aa Protein coding CCDS55338 Q9HC98-3 - TSL:1, GENCODE basic,
NEK6-214 ENST00000545174.5 2573 313aa Protein coding CCDS6854 Q9HC98-1 - TSL:1, GENCODE basic, APPRIS P1,
NEK6-205 ENST00000394199.6 2535 347aa Protein coding CCDS48015 Q9HC98-2 - TSL:2, GENCODE basic,
NEK6-208 ENST00000425237.5 806 222aa Protein coding - Q5TBH1 - CDS 3' incomplete, TSL:3,
NEK6-206 ENST00000422297.5 799 220aa Protein coding - Q5TBH2 - CDS 3' incomplete, TSL:3,
NEK6-209 ENST00000444973.5 790 219aa Protein coding - Q5TBG7 - CDS 3' incomplete, TSL:5,
NEK6-202 ENST00000373596.5 758 207aa Protein coding - Q5TBG1 - CDS 3' incomplete, TSL:3,
NEK6-211 ENST00000454453.5 732 200aa Protein coding - Q5TBH0 - CDS 3' incomplete, TSL:3,
NEK6-207 ENST00000423785.5 726 194aa Protein coding - Q5TBG4 - CDS 3' incomplete, TSL:5,
NEK6-210 ENST00000447379.1 716 171aa Protein coding - Q5TBG2 - CDS 3' incomplete, TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene NEK6
This KO Strategy loading
Red Cotton™ Notes Gene NEK6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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