CRISPR-U™ Gene Knockout Cell Line Strategy

LMAN1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human LMAN1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol LMAN1
Gene id 3998
Organism Homo sapiens
Official full symbol lectin, mannose binding 1
Gene type protein-coding
Also known as ERGIC-53, ERGIC53, F5F8D, FMFD1, MCFD1, MR60, gp58
Summary The protein encoded by this gene is a membrane mannose-specific lectin that cycles between the endoplasmic reticulum, endoplasmic reticulum-Golgi intermediate compartment, and cis-Golgi, functioning as a cargo receptor for glycoprotein transport. The protein has an N-terminal signal sequence, a calcium-dependent and pH-sensitive carbohydrate recognition domain, a stalk region that functions in oligomerization, a transmembrane domain, and a short cytoplasmic domain required for organelle targeting. Allelic variants of this gene are associated with the autosomal recessive disorder combined factor V-factor VIII deficiency.
Genomic regions Chromosome 18
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
LMAN1-201 ENST00000251047.6 4824 510aa Protein coding CCDS11974 A0A024R2A7 P49257 NM_005570.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
LMAN1-204 ENST00000587940.1 442 No protein Processed transcript - - - TSL:2,
LMAN1-202 ENST00000587561.1 1181 No protein Retained intron - - - TSL:2,
LMAN1-203 ENST00000587918.1 678 No protein Retained intron - - - TSL:2,
LMAN1-205 ENST00000592562.1 576 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene LMAN1
This KO Strategy loading
Red Cotton™ Notes Gene LMAN1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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