CRISPR-U™ Gene Knockout Cell Line Strategy

MAPK14 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MAPK14 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MAPK14
Gene id 1432
Organism Homo sapiens
Official full symbol mitogen-activated protein kinase 14
Gene type protein-coding
Also known as CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA
Summary The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Genomic regions Chromosome 6
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MAPK14-202 ENST00000229795.7 4319 360aa Protein coding CCDS4815 Q16539-2 - TSL:1, GENCODE basic, APPRIS ALT1,
MAPK14-201 ENST00000229794.9 4222 360aa Protein coding CCDS4816 Q16539-1 NM_139012.3 TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92,
MAPK14-203 ENST00000310795.8 1066 307aa Protein coding CCDS4817 Q16539-4 - TSL:1, GENCODE basic,
MAPK14-204 ENST00000468133.5 1376 283aa Protein coding - B4E0K5 - TSL:2, GENCODE basic,
MAPK14-210 ENST00000622903.4 1003 173aa Protein coding - B5TY33 - TSL:5, GENCODE basic,
MAPK14-205 ENST00000472333.1 585 132aa Protein coding - E7EX54 - CDS 3' incomplete, TSL:5,
MAPK14-208 ENST00000491957.5 2777 173aa Nonsense mediated decay - B5TY33 - TSL:1,
MAPK14-206 ENST00000474429.5 823 90aa Nonsense mediated decay - H7C4E2 - CDS 5' incomplete, TSL:5,
MAPK14-209 ENST00000496250.5 2750 No protein Retained intron - - - TSL:1,
MAPK14-207 ENST00000490379.1 450 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MAPK14
This KO Strategy loading
Red Cotton™ Notes Gene MAPK14 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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