CRISPR-U™ Gene Knockout Cell Line
Strategy
MAPK14 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MAPK14 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MAPK14 |
| Gene id | 1432 |
| Organism | Homo sapiens |
| Official full symbol | mitogen-activated protein kinase 14 |
| Gene type | protein-coding |
| Also known as | CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA |
| Summary | The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MAPK14-202 | ENST00000229795.7 | 4319 | 360aa | Protein coding | CCDS4815 | Q16539-2 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| MAPK14-201 | ENST00000229794.9 | 4222 | 360aa | Protein coding | CCDS4816 | Q16539-1 | NM_139012.3 | TSL:1, GENCODE basic, APPRIS P3, MANE Select v0.92, |
| MAPK14-203 | ENST00000310795.8 | 1066 | 307aa | Protein coding | CCDS4817 | Q16539-4 | - | TSL:1, GENCODE basic, |
| MAPK14-204 | ENST00000468133.5 | 1376 | 283aa | Protein coding | - | B4E0K5 | - | TSL:2, GENCODE basic, |
| MAPK14-210 | ENST00000622903.4 | 1003 | 173aa | Protein coding | - | B5TY33 | - | TSL:5, GENCODE basic, |
| MAPK14-205 | ENST00000472333.1 | 585 | 132aa | Protein coding | - | E7EX54 | - | CDS 3' incomplete, TSL:5, |
| MAPK14-208 | ENST00000491957.5 | 2777 | 173aa | Nonsense mediated decay | - | B5TY33 | - | TSL:1, |
| MAPK14-206 | ENST00000474429.5 | 823 | 90aa | Nonsense mediated decay | - | H7C4E2 | - | CDS 5' incomplete, TSL:5, |
| MAPK14-209 | ENST00000496250.5 | 2750 | No protein | Retained intron | - | - | - | TSL:1, |
| MAPK14-207 | ENST00000490379.1 | 450 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MAPK14 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MAPK14 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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