CRISPR-U™ Gene Knockout Cell Line
Strategy
ADRM1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ADRM1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ADRM1 |
| Gene id | 11047 |
| Organism | Homo sapiens |
| Official full symbol | adhesion regulating molecule 1 |
| Gene type | protein-coding |
| Also known as | ARM-1, ARM1, GP110 |
| Summary | This gene encodes a member of the adhesion regulating molecule 1 protein family. The encoded protein is a component of the proteasome where it acts as a ubiquitin receptor and recruits the deubiquitinating enzyme, ubiquitin carboxyl-terminal hydrolase L5. Increased levels of the encoded protein are associated with increased cell adhesion, which is likely an indirect effect of this intracellular protein. Dysregulation of this gene has been implicated in carcinogenesis. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 20 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ADRM1-204 | ENST00000491935.5 | 1530 | 407aa | Protein coding | CCDS13496 | Q16186 | - | TSL:5, GENCODE basic, APPRIS P1, |
| ADRM1-201 | ENST00000253003.7 | 1379 | 407aa | Protein coding | CCDS13496 | Q16186 | NM_007002.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ADRM1-205 | ENST00000620230.4 | 1361 | 368aa | Protein coding | CCDS74747 | A0A087WX59 | - | TSL:5, GENCODE basic, |
| ADRM1-202 | ENST00000462554.2 | 735 | 141aa | Protein coding | - | A0A087WUX6 | - | CDS 3' incomplete, TSL:3, |
| ADRM1-203 | ENST00000465805.2 | 630 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ADRM1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ADRM1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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