CRISPR-U™ Gene Knockout Cell Line
Strategy
DLD Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human DLD Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | DLD |
| Gene id | 1738 |
| Organism | Homo sapiens |
| Official full symbol | dihydrolipoamide dehydrogenase |
| Gene type | protein-coding |
| Also known as | DLDD, DLDH, E3, GCSL, LAD, OGDC-E3, PHE3 |
| Summary | This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| DLD-201 | ENST00000205402.10 | 3537 | 509aa | Protein coding | CCDS5749 | P09622-1 | NM_000108.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| DLD-205 | ENST00000440410.5 | 1993 | 486aa | Protein coding | CCDS78269 | E9PEX6 | - | TSL:2, GENCODE basic, |
| DLD-204 | ENST00000437604.6 | 1859 | 461aa | Protein coding | CCDS78268 | P09622-3 | - | TSL:2, GENCODE basic, |
| DLD-214 | ENST00000639772.1 | 421 | 114aa | Protein coding | - | A0A1W2PR83 | - | TSL:5, GENCODE basic, |
| DLD-203 | ENST00000417551.5 | 2041 | 509aa | Nonsense mediated decay | CCDS5749 | P09622-1 | - | TSL:5, |
| DLD-202 | ENST00000415325.5 | 1802 | 58aa | Nonsense mediated decay | - | F8WDY5 | - | TSL:2, |
| DLD-207 | ENST00000451081.5 | 930 | 115aa | Nonsense mediated decay | - | F2Z2E3 | - | TSL:1, |
| DLD-206 | ENST00000450038.5 | 682 | 114aa | Nonsense mediated decay | - | F8WDM5 | - | TSL:5, |
| DLD-213 | ENST00000494441.1 | 707 | No protein | Processed transcript | - | - | - | TSL:3, |
| DLD-208 | ENST00000453354.5 | 537 | No protein | Processed transcript | - | - | - | TSL:4, |
| DLD-212 | ENST00000489184.1 | 638 | No protein | Retained intron | - | - | - | TSL:2, |
| DLD-209 | ENST00000460577.5 | 600 | No protein | Retained intron | - | - | - | TSL:2, |
| DLD-210 | ENST00000478414.1 | 549 | No protein | Retained intron | - | - | - | TSL:4, |
| DLD-211 | ENST00000485066.1 | 483 | No protein | Retained intron | - | - | - | TSL:5, |
Strategy
Click to get
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | DLD |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene DLD had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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