CRISPR-U™ Gene Knockout Cell Line Strategy

DLD Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human DLD Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol DLD
Gene id 1738
Organism Homo sapiens
Official full symbol dihydrolipoamide dehydrogenase
Gene type protein-coding
Also known as DLDD, DLDH, E3, GCSL, LAD, OGDC-E3, PHE3
Summary This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 7
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
DLD-201 ENST00000205402.10 3537 509aa Protein coding CCDS5749 P09622-1 NM_000108.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
DLD-205 ENST00000440410.5 1993 486aa Protein coding CCDS78269 E9PEX6 - TSL:2, GENCODE basic,
DLD-204 ENST00000437604.6 1859 461aa Protein coding CCDS78268 P09622-3 - TSL:2, GENCODE basic,
DLD-214 ENST00000639772.1 421 114aa Protein coding - A0A1W2PR83 - TSL:5, GENCODE basic,
DLD-203 ENST00000417551.5 2041 509aa Nonsense mediated decay CCDS5749 P09622-1 - TSL:5,
DLD-202 ENST00000415325.5 1802 58aa Nonsense mediated decay - F8WDY5 - TSL:2,
DLD-207 ENST00000451081.5 930 115aa Nonsense mediated decay - F2Z2E3 - TSL:1,
DLD-206 ENST00000450038.5 682 114aa Nonsense mediated decay - F8WDM5 - TSL:5,
DLD-213 ENST00000494441.1 707 No protein Processed transcript - - - TSL:3,
DLD-208 ENST00000453354.5 537 No protein Processed transcript - - - TSL:4,
DLD-212 ENST00000489184.1 638 No protein Retained intron - - - TSL:2,
DLD-209 ENST00000460577.5 600 No protein Retained intron - - - TSL:2,
DLD-210 ENST00000478414.1 549 No protein Retained intron - - - TSL:4,
DLD-211 ENST00000485066.1 483 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
Strategy Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene DLD
This KO Strategy loading
Red Cotton™ Notes Gene DLD had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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