CRISPR-U™ Gene Knockout Cell Line Strategy

MPP1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MPP1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MPP1
Gene id 4354
Organism Homo sapiens
Official full symbol membrane palmitoylated protein 1
Gene type protein-coding
Also known as AAG12, DXS552E, EMP55, MRG1, PEMP
Summary This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome X
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MPP1-205 ENST00000413259.7 2195 436aa Protein coding CCDS55544 Q00013-2 - TSL:2, GENCODE basic,
MPP1-202 ENST00000369534.8 2006 466aa Protein coding CCDS14762 Q00013-1 NM_002436.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MPP1-204 ENST00000393531.5 1389 446aa Protein coding CCDS55545 Q00013-3 - TSL:1, GENCODE basic,
MPP1-203 ENST00000393529.5 794 241aa Protein coding - A8MTH1 - CDS 3' incomplete, TSL:5,
MPP1-209 ENST00000453245.5 729 205aa Protein coding - C9J9J4 - CDS 3' incomplete, TSL:5,
MPP1-207 ENST00000428488.1 581 156aa Protein coding - C9JB34 - CDS 3' incomplete, TSL:3,
MPP1-201 ENST00000369531.1 519 131aa Protein coding - A6NFY0 - CDS 3' incomplete, TSL:3,
MPP1-208 ENST00000439370.5 2066 68aa Nonsense mediated decay - F8WC84 - TSL:5,
MPP1-206 ENST00000417435.5 970 89aa Nonsense mediated decay - F8WDV6 - TSL:5,
MPP1-210 ENST00000462825.5 587 No protein Processed transcript - - - TSL:4,
MPP1-211 ENST00000471821.1 558 No protein Processed transcript - - - TSL:2,
MPP1-215 ENST00000488754.5 553 No protein Processed transcript - - - TSL:3,
MPP1-214 ENST00000488694.1 550 No protein Processed transcript - - - TSL:2,
MPP1-216 ENST00000491955.5 2378 No protein Retained intron - - - TSL:2,
MPP1-213 ENST00000482757.1 989 No protein Retained intron - - - TSL:2,
MPP1-218 ENST00000494170.1 969 No protein Retained intron - - - TSL:3,
MPP1-212 ENST00000475943.5 860 No protein Retained intron - - - TSL:1,
MPP1-217 ENST00000493871.1 585 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MPP1
This KO Strategy loading
Red Cotton™ Notes Gene MPP1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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