CRISPR-U™ Gene Knockout Cell Line
Strategy
MPP1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MPP1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MPP1 |
| Gene id | 4354 |
| Organism | Homo sapiens |
| Official full symbol | membrane palmitoylated protein 1 |
| Gene type | protein-coding |
| Also known as | AAG12, DXS552E, EMP55, MRG1, PEMP |
| Summary | This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MPP1-205 | ENST00000413259.7 | 2195 | 436aa | Protein coding | CCDS55544 | Q00013-2 | - | TSL:2, GENCODE basic, |
| MPP1-202 | ENST00000369534.8 | 2006 | 466aa | Protein coding | CCDS14762 | Q00013-1 | NM_002436.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MPP1-204 | ENST00000393531.5 | 1389 | 446aa | Protein coding | CCDS55545 | Q00013-3 | - | TSL:1, GENCODE basic, |
| MPP1-203 | ENST00000393529.5 | 794 | 241aa | Protein coding | - | A8MTH1 | - | CDS 3' incomplete, TSL:5, |
| MPP1-209 | ENST00000453245.5 | 729 | 205aa | Protein coding | - | C9J9J4 | - | CDS 3' incomplete, TSL:5, |
| MPP1-207 | ENST00000428488.1 | 581 | 156aa | Protein coding | - | C9JB34 | - | CDS 3' incomplete, TSL:3, |
| MPP1-201 | ENST00000369531.1 | 519 | 131aa | Protein coding | - | A6NFY0 | - | CDS 3' incomplete, TSL:3, |
| MPP1-208 | ENST00000439370.5 | 2066 | 68aa | Nonsense mediated decay | - | F8WC84 | - | TSL:5, |
| MPP1-206 | ENST00000417435.5 | 970 | 89aa | Nonsense mediated decay | - | F8WDV6 | - | TSL:5, |
| MPP1-210 | ENST00000462825.5 | 587 | No protein | Processed transcript | - | - | - | TSL:4, |
| MPP1-211 | ENST00000471821.1 | 558 | No protein | Processed transcript | - | - | - | TSL:2, |
| MPP1-215 | ENST00000488754.5 | 553 | No protein | Processed transcript | - | - | - | TSL:3, |
| MPP1-214 | ENST00000488694.1 | 550 | No protein | Processed transcript | - | - | - | TSL:2, |
| MPP1-216 | ENST00000491955.5 | 2378 | No protein | Retained intron | - | - | - | TSL:2, |
| MPP1-213 | ENST00000482757.1 | 989 | No protein | Retained intron | - | - | - | TSL:2, |
| MPP1-218 | ENST00000494170.1 | 969 | No protein | Retained intron | - | - | - | TSL:3, |
| MPP1-212 | ENST00000475943.5 | 860 | No protein | Retained intron | - | - | - | TSL:1, |
| MPP1-217 | ENST00000493871.1 | 585 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MPP1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MPP1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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