CRISPR-U™ Gene Knockout Cell Line
Strategy
CD274 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CD274 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CD274 |
| Gene id | 29126 |
| Organism | Homo sapiens |
| Official full symbol | CD274 molecule |
| Gene type | protein-coding |
| Also known as | B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1 |
| Summary | This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 9 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CD274-202 | ENST00000381577.4 | 3634 | 290aa | Protein coding | CCDS6464 | Q9NZQ7-1 | NM_014143.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CD274-201 | ENST00000381573.8 | 3343 | 176aa | Protein coding | CCDS59118 | Q9NZQ7-2 | - | TSL:5, GENCODE basic, |
| CD274-205 | ENST00000498261.1 | 704 | No protein | Processed transcript | - | - | - | TSL:1, |
| CD274-204 | ENST00000492923.1 | 601 | No protein | Processed transcript | - | - | - | TSL:2, |
| CD274-203 | ENST00000474218.1 | 442 | No protein | Processed transcript | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CD274 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CD274 had been KO in a549 cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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