CRISPR-U™ Gene Knockout Cell Line Strategy

CD274 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CD274 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CD274
Gene id 29126
Organism Homo sapiens
Official full symbol CD274 molecule
Gene type protein-coding
Also known as B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, hPD-L1
Summary This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 9
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CD274-202 ENST00000381577.4 3634 290aa Protein coding CCDS6464 Q9NZQ7-1 NM_014143.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CD274-201 ENST00000381573.8 3343 176aa Protein coding CCDS59118 Q9NZQ7-2 - TSL:5, GENCODE basic,
CD274-205 ENST00000498261.1 704 No protein Processed transcript - - - TSL:1,
CD274-204 ENST00000492923.1 601 No protein Processed transcript - - - TSL:2,
CD274-203 ENST00000474218.1 442 No protein Processed transcript - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CD274
This KO Strategy loading
Red Cotton™ Notes Gene CD274 had been KO in a549 cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$39

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×

Comment: