CRISPR-U™ Gene Knockout Cell Line Strategy

ITGA2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ITGA2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ITGA2
Gene id 3673
Organism Homo sapiens
Official full symbol integrin subunit alpha 2
Gene type protein-coding
Also known as BR, CD49B, GPIa, HPA-5, VLA-2, VLAA2
Summary This gene encodes the alpha subunit of a transmembrane receptor for collagens and related proteins. The encoded protein forms a heterodimer with a beta subunit and mediates the adhesion of platelets and other cell types to the extracellular matrix. Loss of the encoded protein is associated with bleeding disorder platelet-type 9. Antibodies against this protein are found in several immune disorders, including neonatal alloimmune thrombocytopenia. This gene is located adjacent to a related alpha subunit gene. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 5
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ITGA2-201 ENST00000296585.10 7843 1181aa Protein coding CCDS3957 P17301 NM_002203.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ITGA2-202 ENST00000503810.6 3782 175aa Nonsense mediated decay - D6RG08 - TSL:5,
ITGA2-204 ENST00000509960.5 3674 641aa Nonsense mediated decay - E9PB77 - TSL:1,
ITGA2-205 ENST00000510722.1 3530 942aa Nonsense mediated decay - E7ESP4 - TSL:1,
ITGA2-206 ENST00000513685.5 3501 54aa Nonsense mediated decay - D6R9H3 - TSL:1,
ITGA2-203 ENST00000509814.5 3487 815aa Nonsense mediated decay - E7EMF1 - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ITGA2
This KO Strategy loading
Red Cotton™ Notes Gene ITGA2 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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