CRISPR-U™ Gene Knockout Cell Line
Strategy
IL18 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IL18 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | IL18 |
| Gene id | 3606 |
| Organism | Homo sapiens |
| Official full symbol | interleukin 18 |
| Gene type | protein-coding |
| Also known as | IGIF, IL-18, IL-1g, IL1F4 |
| Summary | The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 3 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| IL18-201 | ENST00000280357.12 | 1115 | 193aa | Protein coding | CCDS44731 | Q14116-1 | NM_001562.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| IL18-202 | ENST00000524595.5 | 1090 | 189aa | Protein coding | CCDS58180 | Q14116-2 | - | TSL:1, GENCODE basic, |
| IL18-204 | ENST00000528832.1 | 795 | 193aa | Protein coding | CCDS44731 | Q14116-1 | - | TSL:3, GENCODE basic, APPRIS P1, |
| IL18-205 | ENST00000533858.5 | 758 | No protein | Processed transcript | - | - | - | TSL:3, |
| IL18-203 | ENST00000525547.5 | 1697 | No protein | Retained intron | - | - | - | TSL:1, |
| IL18-206 | ENST00000534225.1 | 880 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | IL18 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene IL18 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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