CRISPR-U™ Gene Knockout Cell Line Strategy

IL18 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human IL18 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IL18
Gene id 3606
Organism Homo sapiens
Official full symbol interleukin 18
Gene type protein-coding
Also known as IGIF, IL-18, IL-1g, IL1F4
Summary The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Genomic regions Chromosome 11
Strategy Summary
This gene has 3 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IL18-201 ENST00000280357.12 1115 193aa Protein coding CCDS44731 Q14116-1 NM_001562.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IL18-202 ENST00000524595.5 1090 189aa Protein coding CCDS58180 Q14116-2 - TSL:1, GENCODE basic,
IL18-204 ENST00000528832.1 795 193aa Protein coding CCDS44731 Q14116-1 - TSL:3, GENCODE basic, APPRIS P1,
IL18-205 ENST00000533858.5 758 No protein Processed transcript - - - TSL:3,
IL18-203 ENST00000525547.5 1697 No protein Retained intron - - - TSL:1,
IL18-206 ENST00000534225.1 880 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IL18
This KO Strategy loading
Red Cotton™ Notes Gene IL18 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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