CRISPR-U™ Gene Knockout Cell Line Strategy

CDC25C Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDC25C Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDC25C
Gene id 995
Organism Homo sapiens
Official full symbol cell division cycle 25C
Gene type protein-coding
Also known as CDC25, PPP1R60
Summary This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described.
Genomic regions Chromosome 5
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDC25C-201 ENST00000323760.11 2093 473aa Protein coding CCDS4202 P30307-1 NM_001790.5 TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92,
CDC25C-206 ENST00000513970.5 1968 473aa Protein coding CCDS4202 P30307-1 - TSL:2, GENCODE basic, APPRIS P2,
CDC25C-202 ENST00000415130.6 1556 400aa Protein coding CCDS4203 P30307-4 - TSL:5, GENCODE basic, APPRIS ALT2,
CDC25C-208 ENST00000514555.5 1332 443aa Protein coding - P30307-2 - TSL:5, GENCODE basic, APPRIS ALT2,
CDC25C-203 ENST00000503022.5 1059 249aa Protein coding - D6RJC2 - CDS 3' incomplete, TSL:3,
CDC25C-205 ENST00000510119.1 867 170aa Protein coding - D6RD20 - CDS 3' incomplete, TSL:3,
CDC25C-207 ENST00000514017.1 774 258aa Protein coding - H0Y999 - CDS 5' and 3' incomplete, TSL:5,
CDC25C-209 ENST00000648466.1 2137 232aa Nonsense mediated decay - A0A3B3IT42 - -
CDC25C-204 ENST00000504831.1 536 No protein Retained intron - - - TSL:5,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDC25C
This KO Strategy loading
Red Cotton™ Notes Gene CDC25C had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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