CRISPR-U™ Gene Knockout Cell Line
Strategy
CDC25C Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDC25C Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDC25C |
| Gene id | 995 |
| Organism | Homo sapiens |
| Official full symbol | cell division cycle 25C |
| Gene type | protein-coding |
| Also known as | CDC25, PPP1R60 |
| Summary | This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDC25C-201 | ENST00000323760.11 | 2093 | 473aa | Protein coding | CCDS4202 | P30307-1 | NM_001790.5 | TSL:1, GENCODE basic, APPRIS P2, MANE Select v0.92, |
| CDC25C-206 | ENST00000513970.5 | 1968 | 473aa | Protein coding | CCDS4202 | P30307-1 | - | TSL:2, GENCODE basic, APPRIS P2, |
| CDC25C-202 | ENST00000415130.6 | 1556 | 400aa | Protein coding | CCDS4203 | P30307-4 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| CDC25C-208 | ENST00000514555.5 | 1332 | 443aa | Protein coding | - | P30307-2 | - | TSL:5, GENCODE basic, APPRIS ALT2, |
| CDC25C-203 | ENST00000503022.5 | 1059 | 249aa | Protein coding | - | D6RJC2 | - | CDS 3' incomplete, TSL:3, |
| CDC25C-205 | ENST00000510119.1 | 867 | 170aa | Protein coding | - | D6RD20 | - | CDS 3' incomplete, TSL:3, |
| CDC25C-207 | ENST00000514017.1 | 774 | 258aa | Protein coding | - | H0Y999 | - | CDS 5' and 3' incomplete, TSL:5, |
| CDC25C-209 | ENST00000648466.1 | 2137 | 232aa | Nonsense mediated decay | - | A0A3B3IT42 | - | - |
| CDC25C-204 | ENST00000504831.1 | 536 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDC25C |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDC25C had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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