CRISPR-U™ Gene Knockout Cell Line Strategy

HDAC2 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human HDAC2 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol HDAC2
Gene id 3066
Organism Homo sapiens
Official full symbol histone deacetylase 2
Gene type protein-coding
Also known as HD2, KDAC2, RPD3, YAF1
Summary This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 6
Strategy Summary
This gene has 12 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
HDAC2-205 ENST00000519065.6 9737 488aa Protein coding CCDS43493 Q92769-1 NM_001527.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
HDAC2-201 ENST00000368632.6 2084 458aa Protein coding - Q92769-3 - TSL:2, GENCODE basic,
HDAC2-206 ENST00000519108.5 2000 458aa Protein coding - Q92769-3 - TSL:2, GENCODE basic,
HDAC2-212 ENST00000521610.5 580 100aa Protein coding - E5RH52 - CDS 3' incomplete, TSL:4,
HDAC2-217 ENST00000524334.1 570 52aa Protein coding - E5RK19 - CDS 3' incomplete, TSL:4,
HDAC2-203 ENST00000518690.5 564 71aa Protein coding - E5RGV4 - CDS 3' incomplete, TSL:5,
HDAC2-210 ENST00000521163.5 557 109aa Protein coding - E5RFP9 - CDS 3' incomplete, TSL:5,
HDAC2-214 ENST00000523240.5 557 65aa Protein coding - E5RJ04 - CDS 3' incomplete, TSL:5,
HDAC2-202 ENST00000425835.6 553 161aa Protein coding - H3BM24 - CDS 5' incomplete, TSL:2,
HDAC2-209 ENST00000520895.5 551 60aa Protein coding - E5RFI6 - CDS 3' incomplete, TSL:4,
HDAC2-216 ENST00000523628.5 549 103aa Protein coding - E5RG37 - CDS 3' incomplete, TSL:4,
HDAC2-213 ENST00000522371.5 502 86aa Protein coding - E5RHE7 - CDS 3' incomplete, TSL:4,
HDAC2-215 ENST00000523334.1 4810 No protein Retained intron - - - TSL:2,
HDAC2-204 ENST00000518756.1 826 No protein Retained intron - - - TSL:3,
HDAC2-211 ENST00000521233.1 733 No protein Retained intron - - - TSL:2,
HDAC2-207 ENST00000520170.1 690 No protein Retained intron - - - TSL:2,
HDAC2-208 ENST00000520746.1 497 No protein Retained intron - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene HDAC2
This KO Strategy loading
Red Cotton™ Notes Gene HDAC2 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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