CRISPR-U™ Gene Knockout Cell Line
Strategy
HDAC2 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human HDAC2 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | HDAC2 |
| Gene id | 3066 |
| Organism | Homo sapiens |
| Official full symbol | histone deacetylase 2 |
| Gene type | protein-coding |
| Also known as | HD2, KDAC2, RPD3, YAF1 |
| Summary | This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 6 |
Strategy Summary
This gene has 12 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| HDAC2-205 | ENST00000519065.6 | 9737 | 488aa | Protein coding | CCDS43493 | Q92769-1 | NM_001527.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| HDAC2-201 | ENST00000368632.6 | 2084 | 458aa | Protein coding | - | Q92769-3 | - | TSL:2, GENCODE basic, |
| HDAC2-206 | ENST00000519108.5 | 2000 | 458aa | Protein coding | - | Q92769-3 | - | TSL:2, GENCODE basic, |
| HDAC2-212 | ENST00000521610.5 | 580 | 100aa | Protein coding | - | E5RH52 | - | CDS 3' incomplete, TSL:4, |
| HDAC2-217 | ENST00000524334.1 | 570 | 52aa | Protein coding | - | E5RK19 | - | CDS 3' incomplete, TSL:4, |
| HDAC2-203 | ENST00000518690.5 | 564 | 71aa | Protein coding | - | E5RGV4 | - | CDS 3' incomplete, TSL:5, |
| HDAC2-210 | ENST00000521163.5 | 557 | 109aa | Protein coding | - | E5RFP9 | - | CDS 3' incomplete, TSL:5, |
| HDAC2-214 | ENST00000523240.5 | 557 | 65aa | Protein coding | - | E5RJ04 | - | CDS 3' incomplete, TSL:5, |
| HDAC2-202 | ENST00000425835.6 | 553 | 161aa | Protein coding | - | H3BM24 | - | CDS 5' incomplete, TSL:2, |
| HDAC2-209 | ENST00000520895.5 | 551 | 60aa | Protein coding | - | E5RFI6 | - | CDS 3' incomplete, TSL:4, |
| HDAC2-216 | ENST00000523628.5 | 549 | 103aa | Protein coding | - | E5RG37 | - | CDS 3' incomplete, TSL:4, |
| HDAC2-213 | ENST00000522371.5 | 502 | 86aa | Protein coding | - | E5RHE7 | - | CDS 3' incomplete, TSL:4, |
| HDAC2-215 | ENST00000523334.1 | 4810 | No protein | Retained intron | - | - | - | TSL:2, |
| HDAC2-204 | ENST00000518756.1 | 826 | No protein | Retained intron | - | - | - | TSL:3, |
| HDAC2-211 | ENST00000521233.1 | 733 | No protein | Retained intron | - | - | - | TSL:2, |
| HDAC2-207 | ENST00000520170.1 | 690 | No protein | Retained intron | - | - | - | TSL:2, |
| HDAC2-208 | ENST00000520746.1 | 497 | No protein | Retained intron | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | HDAC2 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene HDAC2 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: