CRISPR-U™ Gene Knockout Cell Line
Strategy
OTUB1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human OTUB1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | OTUB1 |
| Gene id | 55611 |
| Organism | Homo sapiens |
| Official full symbol | OTU deubiquitinase, ubiquitin aldehyde binding 1 |
| Gene type | protein-coding |
| Also known as | HSPC263, OTB1, OTU1 |
| Summary | The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. |
| Genomic regions | Chromosome 11 |
Strategy Summary
This gene has 7 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| OTUB1-209 | ENST00000538426.6 | 1701 | 271aa | Protein coding | CCDS8055 | Q96FW1-1 | NM_017670.3 | TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92, |
| OTUB1-203 | ENST00000428192.6 | 1296 | 271aa | Protein coding | CCDS8055 | Q96FW1-1 | - | TSL:2, GENCODE basic, APPRIS P4, |
| OTUB1-202 | ENST00000422031.6 | 2297 | 272aa | Protein coding | - | J3KR44 | - | TSL:5, GENCODE basic, APPRIS ALT1, |
| OTUB1-210 | ENST00000541478.5 | 1803 | 170aa | Protein coding | - | F5H3F0 | - | TSL:5, GENCODE basic, |
| OTUB1-211 | ENST00000543004.5 | 1647 | 280aa | Protein coding | - | F5H6Q1 | - | TSL:5, GENCODE basic, |
| OTUB1-206 | ENST00000535715.5 | 1391 | 286aa | Protein coding | - | F5GYJ8 | - | TSL:5, GENCODE basic, |
| OTUB1-212 | ENST00000543988.1 | 1259 | 241aa | Protein coding | - | F5GYN4 | - | TSL:1, GENCODE basic, |
| OTUB1-205 | ENST00000535140.1 | 638 | 102aa | Nonsense mediated decay | - | F5H4F3 | - | TSL:3, |
| OTUB1-207 | ENST00000536443.1 | 553 | No protein | Processed transcript | - | - | - | TSL:3, |
| OTUB1-208 | ENST00000536943.1 | 458 | No protein | Processed transcript | - | - | - | TSL:3, |
| OTUB1-201 | ENST00000301453.7 | 2493 | No protein | Retained intron | - | - | - | TSL:2, |
| OTUB1-204 | ENST00000447683.2 | 2315 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | OTUB1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene OTUB1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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