CRISPR-U™ Gene Knockout Cell Line Strategy

OTUB1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human OTUB1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol OTUB1
Gene id 55611
Organism Homo sapiens
Official full symbol OTU deubiquitinase, ubiquitin aldehyde binding 1
Gene type protein-coding
Also known as HSPC263, OTB1, OTU1
Summary The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants.
Genomic regions Chromosome 11
Strategy Summary
This gene has 7 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
OTUB1-209 ENST00000538426.6 1701 271aa Protein coding CCDS8055 Q96FW1-1 NM_017670.3 TSL:1, GENCODE basic, APPRIS P4, MANE Select v0.92,
OTUB1-203 ENST00000428192.6 1296 271aa Protein coding CCDS8055 Q96FW1-1 - TSL:2, GENCODE basic, APPRIS P4,
OTUB1-202 ENST00000422031.6 2297 272aa Protein coding - J3KR44 - TSL:5, GENCODE basic, APPRIS ALT1,
OTUB1-210 ENST00000541478.5 1803 170aa Protein coding - F5H3F0 - TSL:5, GENCODE basic,
OTUB1-211 ENST00000543004.5 1647 280aa Protein coding - F5H6Q1 - TSL:5, GENCODE basic,
OTUB1-206 ENST00000535715.5 1391 286aa Protein coding - F5GYJ8 - TSL:5, GENCODE basic,
OTUB1-212 ENST00000543988.1 1259 241aa Protein coding - F5GYN4 - TSL:1, GENCODE basic,
OTUB1-205 ENST00000535140.1 638 102aa Nonsense mediated decay - F5H4F3 - TSL:3,
OTUB1-207 ENST00000536443.1 553 No protein Processed transcript - - - TSL:3,
OTUB1-208 ENST00000536943.1 458 No protein Processed transcript - - - TSL:3,
OTUB1-201 ENST00000301453.7 2493 No protein Retained intron - - - TSL:2,
OTUB1-204 ENST00000447683.2 2315 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene OTUB1
This KO Strategy loading
Red Cotton™ Notes Gene OTUB1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
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