CRISPR-U™ Gene Knockout Cell Line Strategy

RAB8A Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human RAB8A Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol RAB8A
Gene id 4218
Organism Homo sapiens
Official full symbol RAB8A, member RAS oncogene family
Gene type protein-coding
Also known as MEL, RAB8
Summary The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1.
Genomic regions Chromosome 19
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
RAB8A-201 ENST00000300935.8 2567 207aa Protein coding CCDS12339 P61006-1 NM_005370.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
RAB8A-202 ENST00000586682.1 1315 207aa Protein coding - P61006-2 - TSL:2, GENCODE basic,
RAB8A-204 ENST00000588105.1 520 No protein Processed transcript - - - TSL:4,
RAB8A-205 ENST00000589697.1 1754 No protein Retained intron - - - TSL:1,
RAB8A-206 ENST00000590899.5 578 No protein Retained intron - - - TSL:2,
RAB8A-203 ENST00000587156.5 554 No protein Retained intron - - - TSL:2,
RAB8A-207 ENST00000592971.1 300 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene RAB8A
This KO Strategy loading
Red Cotton™ Notes Gene RAB8A had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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