CRISPR-U™ Gene Knockout Cell Line
Strategy
RAB8A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human RAB8A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | RAB8A |
| Gene id | 4218 |
| Organism | Homo sapiens |
| Official full symbol | RAB8A, member RAS oncogene family |
| Gene type | protein-coding |
| Also known as | MEL, RAB8 |
| Summary | The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. |
| Genomic regions | Chromosome 19 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| RAB8A-201 | ENST00000300935.8 | 2567 | 207aa | Protein coding | CCDS12339 | P61006-1 | NM_005370.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| RAB8A-202 | ENST00000586682.1 | 1315 | 207aa | Protein coding | - | P61006-2 | - | TSL:2, GENCODE basic, |
| RAB8A-204 | ENST00000588105.1 | 520 | No protein | Processed transcript | - | - | - | TSL:4, |
| RAB8A-205 | ENST00000589697.1 | 1754 | No protein | Retained intron | - | - | - | TSL:1, |
| RAB8A-206 | ENST00000590899.5 | 578 | No protein | Retained intron | - | - | - | TSL:2, |
| RAB8A-203 | ENST00000587156.5 | 554 | No protein | Retained intron | - | - | - | TSL:2, |
| RAB8A-207 | ENST00000592971.1 | 300 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | RAB8A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene RAB8A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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