CRISPR-U™ Gene Knockout Cell Line Strategy

ATOX1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATOX1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ATOX1
Gene id 475
Organism Homo sapiens
Official full symbol antioxidant 1 copper chaperone
Gene type protein-coding
Also known as ATX1, HAH1
Summary This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome.
Genomic regions Chromosome 5
Strategy Summary
This gene has 6 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ATOX1-207 ENST00000524142.5 749 68aa Protein coding CCDS47317 O00244 - TSL:5, GENCODE basic, APPRIS P1,
ATOX1-206 ENST00000522710.1 591 68aa Protein coding CCDS47317 O00244 - TSL:2, GENCODE basic, APPRIS P1,
ATOX1-201 ENST00000313115.11 471 68aa Protein coding CCDS47317 O00244 NM_004045.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ATOX1-203 ENST00000521264.5 4341 73aa Protein coding - E5RIM7 - TSL:2, GENCODE basic,
ATOX1-205 ENST00000522314.5 724 59aa Protein coding - E5RGN3 - TSL:2, GENCODE basic,
ATOX1-202 ENST00000520382.5 466 59aa Protein coding - E5RGN3 - TSL:5, GENCODE basic,
ATOX1-204 ENST00000522145.5 423 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ATOX1
This KO Strategy loading
Red Cotton™ Notes Gene ATOX1 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
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