CRISPR-U™ Gene Knockout Cell Line
Strategy
ATOX1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ATOX1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ATOX1 |
| Gene id | 475 |
| Organism | Homo sapiens |
| Official full symbol | antioxidant 1 copper chaperone |
| Gene type | protein-coding |
| Also known as | ATX1, HAH1 |
| Summary | This gene encodes a copper chaperone that plays a role in copper homeostasis by binding and transporting cytosolic copper to ATPase proteins in the trans-Golgi network for later incorporation to the ceruloplasmin. This protein also functions as an antioxidant against superoxide and hydrogen peroxide, and therefore, may play a significant role in cancer carcinogenesis. Because of its cytogenetic location, this gene represents a candidate gene for 5q-syndrome. |
| Genomic regions | Chromosome 5 |
Strategy Summary
This gene has 6 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ATOX1-207 | ENST00000524142.5 | 749 | 68aa | Protein coding | CCDS47317 | O00244 | - | TSL:5, GENCODE basic, APPRIS P1, |
| ATOX1-206 | ENST00000522710.1 | 591 | 68aa | Protein coding | CCDS47317 | O00244 | - | TSL:2, GENCODE basic, APPRIS P1, |
| ATOX1-201 | ENST00000313115.11 | 471 | 68aa | Protein coding | CCDS47317 | O00244 | NM_004045.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ATOX1-203 | ENST00000521264.5 | 4341 | 73aa | Protein coding | - | E5RIM7 | - | TSL:2, GENCODE basic, |
| ATOX1-205 | ENST00000522314.5 | 724 | 59aa | Protein coding | - | E5RGN3 | - | TSL:2, GENCODE basic, |
| ATOX1-202 | ENST00000520382.5 | 466 | 59aa | Protein coding | - | E5RGN3 | - | TSL:5, GENCODE basic, |
| ATOX1-204 | ENST00000522145.5 | 423 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ATOX1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ATOX1 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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