CRISPR-U™ Gene Knockout Cell Line
Strategy
USP7 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human USP7 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | USP7 |
| Gene id | 7874 |
| Organism | Homo sapiens |
| Official full symbol | ubiquitin specific peptidase 7 |
| Gene type | protein-coding |
| Also known as | HAFOUS, HAUSP, TEF1 |
| Summary | The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder. |
| Genomic regions | Chromosome 16 |
Strategy Summary
This gene has 8 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| USP7-201 | ENST00000344836.9 | 5831 | 1102aa | Protein coding | CCDS32385 | Q93009-1 | NM_003470.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| USP7-202 | ENST00000381886.8 | 3587 | 1086aa | Protein coding | CCDS66941 | Q93009-3 | - | TSL:1, GENCODE basic, |
| USP7-222 | ENST00000673704.1 | 5366 | 1137aa | Protein coding | - | A0A669KBL1 | - | GENCODE basic, |
| USP7-207 | ENST00000563085.5 | 3194 | 909aa | Protein coding | - | H3BND8 | - | CDS 3' incomplete, TSL:2, |
| USP7-215 | ENST00000566273.5 | 577 | 159aa | Protein coding | - | H3BQD1 | - | CDS 3' incomplete, TSL:4, |
| USP7-219 | ENST00000569230.5 | 569 | 117aa | Protein coding | - | H3BTM1 | - | CDS 3' incomplete, TSL:5, |
| USP7-212 | ENST00000566004.5 | 559 | 130aa | Protein coding | - | H3BRA2 | - | CDS 3' incomplete, TSL:4, |
| USP7-209 | ENST00000564117.1 | 479 | 109aa | Protein coding | - | H3BUV0 | - | CDS 3' incomplete, TSL:4, |
| USP7-208 | ENST00000563961.5 | 4533 | 91aa | Nonsense mediated decay | - | H3BMF6 | - | TSL:5, |
| USP7-210 | ENST00000565455.5 | 3554 | 47aa | Nonsense mediated decay | - | H3BRI4 | - | TSL:2, |
| USP7-203 | ENST00000542333.5 | 1966 | 509aa | Nonsense mediated decay | - | F5H2X1 | - | TSL:2, |
| USP7-206 | ENST00000563043.1 | 332 | 68aa | Nonsense mediated decay | - | I3L2D8 | - | CDS 5' incomplete, TSL:5, |
| USP7-218 | ENST00000567692.1 | 313 | 17aa | Nonsense mediated decay | - | H3BVA7 | - | CDS 5' incomplete, TSL:5, |
| USP7-214 | ENST00000566224.1 | 503 | No protein | Processed transcript | - | - | - | TSL:3, |
| USP7-220 | ENST00000569448.1 | 698 | No protein | Retained intron | - | - | - | TSL:2, |
| USP7-221 | ENST00000570256.5 | 591 | No protein | Retained intron | - | - | - | TSL:3, |
| USP7-213 | ENST00000566131.1 | 569 | No protein | Retained intron | - | - | - | TSL:2, |
| USP7-204 | ENST00000562051.1 | 567 | No protein | Retained intron | - | - | - | TSL:4, |
| USP7-217 | ENST00000567329.1 | 567 | No protein | Retained intron | - | - | - | TSL:4, |
| USP7-211 | ENST00000565883.1 | 453 | No protein | Retained intron | - | - | - | TSL:2, |
| USP7-216 | ENST00000567113.5 | 448 | No protein | Retained intron | - | - | - | TSL:2, |
| USP7-205 | ENST00000562615.1 | 304 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | USP7 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene USP7 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$39
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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