CRISPR-U™ Gene Knockout Cell Line Strategy

USP7 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human USP7 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol USP7
Gene id 7874
Organism Homo sapiens
Official full symbol ubiquitin specific peptidase 7
Gene type protein-coding
Also known as HAFOUS, HAUSP, TEF1
Summary The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder.
Genomic regions Chromosome 16
Strategy Summary
This gene has 8 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
USP7-201 ENST00000344836.9 5831 1102aa Protein coding CCDS32385 Q93009-1 NM_003470.3 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
USP7-202 ENST00000381886.8 3587 1086aa Protein coding CCDS66941 Q93009-3 - TSL:1, GENCODE basic,
USP7-222 ENST00000673704.1 5366 1137aa Protein coding - A0A669KBL1 - GENCODE basic,
USP7-207 ENST00000563085.5 3194 909aa Protein coding - H3BND8 - CDS 3' incomplete, TSL:2,
USP7-215 ENST00000566273.5 577 159aa Protein coding - H3BQD1 - CDS 3' incomplete, TSL:4,
USP7-219 ENST00000569230.5 569 117aa Protein coding - H3BTM1 - CDS 3' incomplete, TSL:5,
USP7-212 ENST00000566004.5 559 130aa Protein coding - H3BRA2 - CDS 3' incomplete, TSL:4,
USP7-209 ENST00000564117.1 479 109aa Protein coding - H3BUV0 - CDS 3' incomplete, TSL:4,
USP7-208 ENST00000563961.5 4533 91aa Nonsense mediated decay - H3BMF6 - TSL:5,
USP7-210 ENST00000565455.5 3554 47aa Nonsense mediated decay - H3BRI4 - TSL:2,
USP7-203 ENST00000542333.5 1966 509aa Nonsense mediated decay - F5H2X1 - TSL:2,
USP7-206 ENST00000563043.1 332 68aa Nonsense mediated decay - I3L2D8 - CDS 5' incomplete, TSL:5,
USP7-218 ENST00000567692.1 313 17aa Nonsense mediated decay - H3BVA7 - CDS 5' incomplete, TSL:5,
USP7-214 ENST00000566224.1 503 No protein Processed transcript - - - TSL:3,
USP7-220 ENST00000569448.1 698 No protein Retained intron - - - TSL:2,
USP7-221 ENST00000570256.5 591 No protein Retained intron - - - TSL:3,
USP7-213 ENST00000566131.1 569 No protein Retained intron - - - TSL:2,
USP7-204 ENST00000562051.1 567 No protein Retained intron - - - TSL:4,
USP7-217 ENST00000567329.1 567 No protein Retained intron - - - TSL:4,
USP7-211 ENST00000565883.1 453 No protein Retained intron - - - TSL:2,
USP7-216 ENST00000567113.5 448 No protein Retained intron - - - TSL:2,
USP7-205 ENST00000562615.1 304 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene USP7
This KO Strategy loading
Red Cotton™ Notes Gene USP7 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×