CRISPR-U™ Gene Knockout Cell Line
Strategy
CAPG Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CAPG Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CAPG |
| Gene id | 822 |
| Organism | Homo sapiens |
| Official full symbol | capping actin protein, gelsolin like |
| Gene type | protein-coding |
| Also known as | AFCP, HEL-S-66, MCP |
| Summary | This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene. |
| Genomic regions | Chromosome 2 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CAPG-201 | ENST00000263867.9 | 1250 | 348aa | Protein coding | CCDS1974 | P40121-1 | NM_001747.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CAPG-205 | ENST00000409921.5 | 1195 | 333aa | Protein coding | CCDS58715 | P40121-2 | - | TSL:1, GENCODE basic, |
| CAPG-203 | ENST00000409670.5 | 1183 | 348aa | Protein coding | CCDS1974 | P40121-1 | - | TSL:5, GENCODE basic, APPRIS P1, |
| CAPG-204 | ENST00000409724.5 | 1151 | 348aa | Protein coding | CCDS1974 | P40121-1 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CAPG-208 | ENST00000447219.6 | 930 | 253aa | Protein coding | - | E7ENU9 | - | CDS 3' incomplete, TSL:5, |
| CAPG-207 | ENST00000439385.5 | 865 | 253aa | Protein coding | - | E7ENU9 | - | CDS 3' incomplete, TSL:5, |
| CAPG-209 | ENST00000449030.5 | 839 | 253aa | Protein coding | - | E7ENU9 | - | CDS 3' incomplete, TSL:2, |
| CAPG-202 | ENST00000409275.1 | 835 | 218aa | Protein coding | - | B8ZZL6 | - | CDS 3' incomplete, TSL:3, |
| CAPG-210 | ENST00000453973.5 | 452 | 103aa | Protein coding | - | H7C0X8 | - | CDS 5' incomplete, TSL:3, |
| CAPG-214 | ENST00000483659.5 | 767 | No protein | Processed transcript | - | - | - | TSL:3, |
| CAPG-206 | ENST00000415012.1 | 531 | No protein | Processed transcript | - | - | - | TSL:3, |
| CAPG-213 | ENST00000479651.1 | 265 | No protein | Processed transcript | - | - | - | TSL:5, |
| CAPG-211 | ENST00000459793.2 | 943 | No protein | Retained intron | - | - | - | TSL:5, |
| CAPG-215 | ENST00000491267.1 | 549 | No protein | Retained intron | - | - | - | TSL:2, |
| CAPG-212 | ENST00000471064.1 | 486 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CAPG |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CAPG had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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