CRISPR-U™ Gene Knockout Cell Line Strategy

CAPG Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CAPG Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CAPG
Gene id 822
Organism Homo sapiens
Official full symbol capping actin protein, gelsolin like
Gene type protein-coding
Also known as AFCP, HEL-S-66, MCP
Summary This gene encodes a member of the gelsolin/villin family of actin-regulatory proteins. The encoded protein reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, the encoded protein contributes to the control of actin-based motility in non-muscle cells. Alternatively spliced transcript variants have been observed for this gene.
Genomic regions Chromosome 2
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CAPG-201 ENST00000263867.9 1250 348aa Protein coding CCDS1974 P40121-1 NM_001747.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CAPG-205 ENST00000409921.5 1195 333aa Protein coding CCDS58715 P40121-2 - TSL:1, GENCODE basic,
CAPG-203 ENST00000409670.5 1183 348aa Protein coding CCDS1974 P40121-1 - TSL:5, GENCODE basic, APPRIS P1,
CAPG-204 ENST00000409724.5 1151 348aa Protein coding CCDS1974 P40121-1 - TSL:1, GENCODE basic, APPRIS P1,
CAPG-208 ENST00000447219.6 930 253aa Protein coding - E7ENU9 - CDS 3' incomplete, TSL:5,
CAPG-207 ENST00000439385.5 865 253aa Protein coding - E7ENU9 - CDS 3' incomplete, TSL:5,
CAPG-209 ENST00000449030.5 839 253aa Protein coding - E7ENU9 - CDS 3' incomplete, TSL:2,
CAPG-202 ENST00000409275.1 835 218aa Protein coding - B8ZZL6 - CDS 3' incomplete, TSL:3,
CAPG-210 ENST00000453973.5 452 103aa Protein coding - H7C0X8 - CDS 5' incomplete, TSL:3,
CAPG-214 ENST00000483659.5 767 No protein Processed transcript - - - TSL:3,
CAPG-206 ENST00000415012.1 531 No protein Processed transcript - - - TSL:3,
CAPG-213 ENST00000479651.1 265 No protein Processed transcript - - - TSL:5,
CAPG-211 ENST00000459793.2 943 No protein Retained intron - - - TSL:5,
CAPG-215 ENST00000491267.1 549 No protein Retained intron - - - TSL:2,
CAPG-212 ENST00000471064.1 486 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CAPG
This KO Strategy loading
Red Cotton™ Notes Gene CAPG had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
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Work flow
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