CRISPR-U™ Gene Knockout Cell Line Strategy

GRN Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human GRN Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol GRN
Gene id 2896
Organism Homo sapiens
Official full symbol granulin precursor
Gene type protein-coding
Also known as CLN11, GEP, GP88, PCDGF, PEPI, PGRN
Summary Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.
Genomic regions Chromosome 17
Strategy Summary
This gene has 15 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
GRN-201 ENST00000053867.8 2130 593aa Protein coding CCDS11483 P28799-1 NM_002087.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
GRN-214 ENST00000589265.5 1632 436aa Protein coding - K7EQ05 - TSL:5, GENCODE basic,
GRN-205 ENST00000586443.1 1557 388aa Protein coding - K7EKL3 - CDS 5' incomplete, TSL:3,
GRN-222 ENST00000639447.1 1314 438aa Protein coding - P28799-2 - TSL:2, GENCODE basic,
GRN-221 ENST00000593167.5 763 149aa Protein coding - K7EM89 - CDS 3' incomplete, TSL:5,
GRN-204 ENST00000586242.1 665 110aa Protein coding - K7EQI0 - CDS 5' incomplete, TSL:3,
GRN-208 ENST00000587387.5 614 191aa Protein coding - K7EPL0 - CDS 3' incomplete, TSL:4,
GRN-218 ENST00000591740.5 585 135aa Protein coding - K7ENN1 - CDS 3' incomplete, TSL:4,
GRN-203 ENST00000585512.5 562 37aa Protein coding - K7EQA7 - CDS 3' incomplete, TSL:4,
GRN-213 ENST00000588237.5 558 172aa Protein coding - K7EMR1 - CDS 3' incomplete, TSL:5,
GRN-211 ENST00000588143.5 546 163aa Protein coding - K7EJY4 - CDS 3' incomplete, TSL:3,
GRN-215 ENST00000589536.5 546 111aa Protein coding - K7ENI2 - CDS 3' incomplete, TSL:3,
GRN-220 ENST00000592783.5 541 142aa Protein coding - K7EQK6 - CDS 3' incomplete, TSL:4,
GRN-207 ENST00000587109.5 535 62aa Protein coding - K7ELY1 - CDS 3' incomplete, TSL:2,
GRN-209 ENST00000587518.5 526 136aa Protein coding - K7EK92 - CDS 3' incomplete, TSL:4,
GRN-206 ENST00000586782.5 843 189aa Nonsense mediated decay - K7ERM2 - TSL:5,
GRN-216 ENST00000589923.1 437 No protein Processed transcript - - - TSL:2,
GRN-202 ENST00000585348.1 600 No protein Retained intron - - - TSL:2,
GRN-212 ENST00000588170.5 588 No protein Retained intron - - - TSL:2,
GRN-217 ENST00000590984.1 562 No protein Retained intron - - - TSL:2,
GRN-210 ENST00000587958.1 560 No protein Retained intron - - - TSL:2,
GRN-219 ENST00000592323.5 550 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
Click to get
Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene GRN
This KO Strategy loading
Red Cotton™ Notes Gene GRN had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

Please leave your suggestion ×