CRISPR-U™ Gene Knockout Cell Line
Strategy
GRN Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human GRN Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | GRN |
| Gene id | 2896 |
| Organism | Homo sapiens |
| Official full symbol | granulin precursor |
| Gene type | protein-coding |
| Also known as | CLN11, GEP, GP88, PCDGF, PEPI, PGRN |
| Summary | Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. |
| Genomic regions | Chromosome 17 |
Strategy Summary
This gene has 15 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| GRN-201 | ENST00000053867.8 | 2130 | 593aa | Protein coding | CCDS11483 | P28799-1 | NM_002087.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| GRN-214 | ENST00000589265.5 | 1632 | 436aa | Protein coding | - | K7EQ05 | - | TSL:5, GENCODE basic, |
| GRN-205 | ENST00000586443.1 | 1557 | 388aa | Protein coding | - | K7EKL3 | - | CDS 5' incomplete, TSL:3, |
| GRN-222 | ENST00000639447.1 | 1314 | 438aa | Protein coding | - | P28799-2 | - | TSL:2, GENCODE basic, |
| GRN-221 | ENST00000593167.5 | 763 | 149aa | Protein coding | - | K7EM89 | - | CDS 3' incomplete, TSL:5, |
| GRN-204 | ENST00000586242.1 | 665 | 110aa | Protein coding | - | K7EQI0 | - | CDS 5' incomplete, TSL:3, |
| GRN-208 | ENST00000587387.5 | 614 | 191aa | Protein coding | - | K7EPL0 | - | CDS 3' incomplete, TSL:4, |
| GRN-218 | ENST00000591740.5 | 585 | 135aa | Protein coding | - | K7ENN1 | - | CDS 3' incomplete, TSL:4, |
| GRN-203 | ENST00000585512.5 | 562 | 37aa | Protein coding | - | K7EQA7 | - | CDS 3' incomplete, TSL:4, |
| GRN-213 | ENST00000588237.5 | 558 | 172aa | Protein coding | - | K7EMR1 | - | CDS 3' incomplete, TSL:5, |
| GRN-211 | ENST00000588143.5 | 546 | 163aa | Protein coding | - | K7EJY4 | - | CDS 3' incomplete, TSL:3, |
| GRN-215 | ENST00000589536.5 | 546 | 111aa | Protein coding | - | K7ENI2 | - | CDS 3' incomplete, TSL:3, |
| GRN-220 | ENST00000592783.5 | 541 | 142aa | Protein coding | - | K7EQK6 | - | CDS 3' incomplete, TSL:4, |
| GRN-207 | ENST00000587109.5 | 535 | 62aa | Protein coding | - | K7ELY1 | - | CDS 3' incomplete, TSL:2, |
| GRN-209 | ENST00000587518.5 | 526 | 136aa | Protein coding | - | K7EK92 | - | CDS 3' incomplete, TSL:4, |
| GRN-206 | ENST00000586782.5 | 843 | 189aa | Nonsense mediated decay | - | K7ERM2 | - | TSL:5, |
| GRN-216 | ENST00000589923.1 | 437 | No protein | Processed transcript | - | - | - | TSL:2, |
| GRN-202 | ENST00000585348.1 | 600 | No protein | Retained intron | - | - | - | TSL:2, |
| GRN-212 | ENST00000588170.5 | 588 | No protein | Retained intron | - | - | - | TSL:2, |
| GRN-217 | ENST00000590984.1 | 562 | No protein | Retained intron | - | - | - | TSL:2, |
| GRN-210 | ENST00000587958.1 | 560 | No protein | Retained intron | - | - | - | TSL:2, |
| GRN-219 | ENST00000592323.5 | 550 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | GRN |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene GRN had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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