CRISPR-U™ Gene Knockout Cell Line
Strategy
KDM6A Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KDM6A Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KDM6A |
| Gene id | 7403 |
| Organism | Homo sapiens |
| Official full symbol | lysine demethylase 6A |
| Gene type | protein-coding |
| Also known as | KABUK2, UTX, bA386N14.2 |
| Summary | This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 11 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KDM6A-201 | ENST00000377967.9 | 5761 | 1401aa | Protein coding | CCDS14265 | O15550 | - | TSL:1, GENCODE basic, APPRIS P4, |
| KDM6A-211 | ENST00000611820.5 | 6094 | 1453aa | Protein coding | - | A0A087X0R0 | NM_001291415.2 | TSL:1, GENCODE basic, APPRIS ALT1, MANE Select v0.92, |
| KDM6A-215 | ENST00000674586.1 | 5779 | 1427aa | Protein coding | - | - | - | GENCODE basic, |
| KDM6A-202 | ENST00000382899.9 | 5778 | 1408aa | Protein coding | - | F8W8R6 | - | TSL:1, GENCODE basic, APPRIS ALT1, |
| KDM6A-209 | ENST00000536777.6 | 5622 | 1356aa | Protein coding | - | F5H6S1 | - | TSL:1, GENCODE basic, |
| KDM6A-225 | ENST00000675577.1 | 5599 | 1367aa | Protein coding | - | - | - | APPRIS ALT1, |
| KDM6A-218 | ENST00000674867.1 | 5563 | 1355aa | Protein coding | - | - | - | GENCODE basic, |
| KDM6A-210 | ENST00000543216.6 | 5520 | 1322aa | Protein coding | - | F5H5V6 | - | TSL:1, GENCODE basic, |
| KDM6A-203 | ENST00000414389.5 | 4189 | 999aa | Protein coding | - | H0Y6I7 | - | CDS 5' incomplete, TSL:5, |
| KDM6A-212 | ENST00000621147.4 | 2876 | 224aa | Protein coding | - | A0A087WUN6 | - | TSL:1, GENCODE basic, |
| KDM6A-204 | ENST00000431196.3 | 1675 | 161aa | Protein coding | - | H0Y6V5 | - | CDS 5' incomplete, TSL:3, |
| KDM6A-230 | ENST00000676133.1 | 6135 | 126aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-228 | ENST00000676062.1 | 5677 | 1336aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-214 | ENST00000674564.1 | 5644 | 1293aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-222 | ENST00000675514.1 | 5642 | 1129aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-213 | ENST00000674541.1 | 5505 | 151aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-216 | ENST00000674659.1 | 5395 | 151aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-231 | ENST00000676343.1 | 5363 | 1256aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-229 | ENST00000676085.1 | 5343 | 151aa | Nonsense mediated decay | - | - | - | - |
| KDM6A-224 | ENST00000675546.1 | 12117 | No protein | Retained intron | - | - | - | - |
| KDM6A-223 | ENST00000675525.1 | 7459 | No protein | Retained intron | - | - | - | - |
| KDM6A-217 | ENST00000674739.1 | 6395 | No protein | Retained intron | - | - | - | - |
| KDM6A-226 | ENST00000675816.1 | 6164 | No protein | Retained intron | - | - | - | - |
| KDM6A-232 | ENST00000676389.1 | 5780 | No protein | Retained intron | - | - | - | - |
| KDM6A-220 | ENST00000675182.1 | 5377 | No protein | Retained intron | - | - | - | - |
| KDM6A-219 | ENST00000675157.1 | 4944 | No protein | Retained intron | - | - | - | - |
| KDM6A-221 | ENST00000675440.1 | 4634 | No protein | Retained intron | - | - | - | - |
| KDM6A-206 | ENST00000479423.2 | 4428 | No protein | Retained intron | - | - | - | TSL:3, |
| KDM6A-227 | ENST00000676026.1 | 2316 | No protein | Retained intron | - | - | - | - |
| KDM6A-207 | ENST00000484732.1 | 640 | No protein | Retained intron | - | - | - | TSL:2, |
| KDM6A-205 | ENST00000475233.1 | 612 | No protein | Retained intron | - | - | - | TSL:5, |
| KDM6A-208 | ENST00000485072.5 | 382 | No protein | Retained intron | - | - | - | TSL:5, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KDM6A |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KDM6A had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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