CRISPR-U™ Gene Knockout Cell Line Strategy

MLH1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human MLH1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol MLH1
Gene id 4292
Organism Homo sapiens
Official full symbol mutL homolog 1
Gene type protein-coding
Also known as COCA2, FCC2, HNPCC, HNPCC2, hMLH1
Summary The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).
Genomic regions Chromosome 3
Strategy Summary
This gene has 16 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
MLH1-216 ENST00000458205.6 2608 515aa Protein coding CCDS54563 P40692-2 - TSL:1, GENCODE basic,
MLH1-235 ENST00000674019.1 2576 515aa Protein coding CCDS54563 P40692-2 - GENCODE basic,
MLH1-221 ENST00000536378.5 2567 515aa Protein coding CCDS54563 P40692-2 - TSL:2, GENCODE basic,
MLH1-201 ENST00000231790.8 2494 756aa Protein coding CCDS2663 P40692-1 NM_000249.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
MLH1-206 ENST00000435176.5 2428 658aa Protein coding CCDS54562 P40692-3 - TSL:2, GENCODE basic,
MLH1-212 ENST00000455445.6 2296 515aa Protein coding CCDS54563 P40692-2 - TSL:2, GENCODE basic,
MLH1-222 ENST00000539477.6 2280 515aa Protein coding CCDS54563 P40692-2 - TSL:2, GENCODE basic,
MLH1-213 ENST00000456676.6 2230 679aa Protein coding - H0Y818 - CDS 5' incomplete, TSL:1,
MLH1-224 ENST00000673673.1 2205 686aa Protein coding - A0A669KAW3 - CDS 5' incomplete,
MLH1-207 ENST00000441265.6 1982 327aa Protein coding - E7EUC9 - TSL:5, GENCODE basic,
MLH1-227 ENST00000673715.1 1978 633aa Protein coding - A0A669KBK2 - GENCODE basic,
MLH1-231 ENST00000673899.1 1712 512aa Protein coding - A0A669KB03 - GENCODE basic,
MLH1-223 ENST00000616768.5 1408 404aa Protein coding - A0A087WX20 - CDS 5' incomplete, TSL:2,
MLH1-204 ENST00000429117.5 562 83aa Protein coding - C9JZ54 - CDS 3' incomplete, TSL:4,
MLH1-203 ENST00000413740.1 342 114aa Protein coding - H0Y806 - CDS 5' and 3' incomplete, TSL:1,
MLH1-210 ENST00000450420.5 233 61aa Protein coding - H0Y4N0 - CDS 5' incomplete, TSL:1,
MLH1-232 ENST00000673947.1 2698 38aa Nonsense mediated decay - F2Z298 - -
MLH1-233 ENST00000673972.1 2473 38aa Nonsense mediated decay - F2Z298 - -
MLH1-205 ENST00000432299.6 2444 48aa Nonsense mediated decay - E9PF25 - TSL:1,
MLH1-237 ENST00000674111.1 2403 568aa Nonsense mediated decay - A0A669KBB4 - -
MLH1-230 ENST00000673897.1 2338 38aa Nonsense mediated decay - F2Z298 - -
MLH1-209 ENST00000447829.6 2247 143aa Nonsense mediated decay - H0Y5L7 - TSL:1,
MLH1-202 ENST00000413212.2 2183 36aa Nonsense mediated decay - H0Y5U4 - TSL:5,
MLH1-214 ENST00000457004.5 668 38aa Nonsense mediated decay - F2Z298 - TSL:3,
MLH1-215 ENST00000458009.5 660 95aa Nonsense mediated decay - H0Y793 - CDS 5' incomplete, TSL:1,
MLH1-211 ENST00000454028.5 570 38aa Nonsense mediated decay - F2Z298 - TSL:5,
MLH1-234 ENST00000673990.1 1711 No protein Processed transcript - - - -
MLH1-220 ENST00000492474.5 589 No protein Processed transcript - - - TSL:5,
MLH1-219 ENST00000485889.1 566 No protein Processed transcript - - - TSL:5,
MLH1-217 ENST00000466900.5 555 No protein Processed transcript - - - TSL:4,
MLH1-236 ENST00000674107.1 2827 No protein Retained intron - - - -
MLH1-229 ENST00000673889.1 1799 No protein Retained intron - - - -
MLH1-226 ENST00000673713.1 1586 No protein Retained intron - - - -
MLH1-228 ENST00000673741.1 1495 No protein Retained intron - - - -
MLH1-208 ENST00000442249.6 1303 No protein Retained intron - - - TSL:4,
MLH1-218 ENST00000476172.6 1246 No protein Retained intron - - - TSL:3,
MLH1-238 ENST00000674125.1 1167 No protein Retained intron - - - -
MLH1-225 ENST00000673686.1 1127 No protein Retained intron - - - -
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene MLH1
This KO Strategy loading
Red Cotton™ Notes Gene MLH1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


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Single gRNA lentivirus

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