CRISPR-U™ Gene Knockout Cell Line
Strategy
MLH1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human MLH1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | MLH1 |
| Gene id | 4292 |
| Organism | Homo sapiens |
| Official full symbol | mutL homolog 1 |
| Gene type | protein-coding |
| Also known as | COCA2, FCC2, HNPCC, HNPCC2, hMLH1 |
| Summary | The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). |
| Genomic regions | Chromosome 3 |
Strategy Summary
This gene has 16 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| MLH1-216 | ENST00000458205.6 | 2608 | 515aa | Protein coding | CCDS54563 | P40692-2 | - | TSL:1, GENCODE basic, |
| MLH1-235 | ENST00000674019.1 | 2576 | 515aa | Protein coding | CCDS54563 | P40692-2 | - | GENCODE basic, |
| MLH1-221 | ENST00000536378.5 | 2567 | 515aa | Protein coding | CCDS54563 | P40692-2 | - | TSL:2, GENCODE basic, |
| MLH1-201 | ENST00000231790.8 | 2494 | 756aa | Protein coding | CCDS2663 | P40692-1 | NM_000249.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| MLH1-206 | ENST00000435176.5 | 2428 | 658aa | Protein coding | CCDS54562 | P40692-3 | - | TSL:2, GENCODE basic, |
| MLH1-212 | ENST00000455445.6 | 2296 | 515aa | Protein coding | CCDS54563 | P40692-2 | - | TSL:2, GENCODE basic, |
| MLH1-222 | ENST00000539477.6 | 2280 | 515aa | Protein coding | CCDS54563 | P40692-2 | - | TSL:2, GENCODE basic, |
| MLH1-213 | ENST00000456676.6 | 2230 | 679aa | Protein coding | - | H0Y818 | - | CDS 5' incomplete, TSL:1, |
| MLH1-224 | ENST00000673673.1 | 2205 | 686aa | Protein coding | - | A0A669KAW3 | - | CDS 5' incomplete, |
| MLH1-207 | ENST00000441265.6 | 1982 | 327aa | Protein coding | - | E7EUC9 | - | TSL:5, GENCODE basic, |
| MLH1-227 | ENST00000673715.1 | 1978 | 633aa | Protein coding | - | A0A669KBK2 | - | GENCODE basic, |
| MLH1-231 | ENST00000673899.1 | 1712 | 512aa | Protein coding | - | A0A669KB03 | - | GENCODE basic, |
| MLH1-223 | ENST00000616768.5 | 1408 | 404aa | Protein coding | - | A0A087WX20 | - | CDS 5' incomplete, TSL:2, |
| MLH1-204 | ENST00000429117.5 | 562 | 83aa | Protein coding | - | C9JZ54 | - | CDS 3' incomplete, TSL:4, |
| MLH1-203 | ENST00000413740.1 | 342 | 114aa | Protein coding | - | H0Y806 | - | CDS 5' and 3' incomplete, TSL:1, |
| MLH1-210 | ENST00000450420.5 | 233 | 61aa | Protein coding | - | H0Y4N0 | - | CDS 5' incomplete, TSL:1, |
| MLH1-232 | ENST00000673947.1 | 2698 | 38aa | Nonsense mediated decay | - | F2Z298 | - | - |
| MLH1-233 | ENST00000673972.1 | 2473 | 38aa | Nonsense mediated decay | - | F2Z298 | - | - |
| MLH1-205 | ENST00000432299.6 | 2444 | 48aa | Nonsense mediated decay | - | E9PF25 | - | TSL:1, |
| MLH1-237 | ENST00000674111.1 | 2403 | 568aa | Nonsense mediated decay | - | A0A669KBB4 | - | - |
| MLH1-230 | ENST00000673897.1 | 2338 | 38aa | Nonsense mediated decay | - | F2Z298 | - | - |
| MLH1-209 | ENST00000447829.6 | 2247 | 143aa | Nonsense mediated decay | - | H0Y5L7 | - | TSL:1, |
| MLH1-202 | ENST00000413212.2 | 2183 | 36aa | Nonsense mediated decay | - | H0Y5U4 | - | TSL:5, |
| MLH1-214 | ENST00000457004.5 | 668 | 38aa | Nonsense mediated decay | - | F2Z298 | - | TSL:3, |
| MLH1-215 | ENST00000458009.5 | 660 | 95aa | Nonsense mediated decay | - | H0Y793 | - | CDS 5' incomplete, TSL:1, |
| MLH1-211 | ENST00000454028.5 | 570 | 38aa | Nonsense mediated decay | - | F2Z298 | - | TSL:5, |
| MLH1-234 | ENST00000673990.1 | 1711 | No protein | Processed transcript | - | - | - | - |
| MLH1-220 | ENST00000492474.5 | 589 | No protein | Processed transcript | - | - | - | TSL:5, |
| MLH1-219 | ENST00000485889.1 | 566 | No protein | Processed transcript | - | - | - | TSL:5, |
| MLH1-217 | ENST00000466900.5 | 555 | No protein | Processed transcript | - | - | - | TSL:4, |
| MLH1-236 | ENST00000674107.1 | 2827 | No protein | Retained intron | - | - | - | - |
| MLH1-229 | ENST00000673889.1 | 1799 | No protein | Retained intron | - | - | - | - |
| MLH1-226 | ENST00000673713.1 | 1586 | No protein | Retained intron | - | - | - | - |
| MLH1-228 | ENST00000673741.1 | 1495 | No protein | Retained intron | - | - | - | - |
| MLH1-208 | ENST00000442249.6 | 1303 | No protein | Retained intron | - | - | - | TSL:4, |
| MLH1-218 | ENST00000476172.6 | 1246 | No protein | Retained intron | - | - | - | TSL:3, |
| MLH1-238 | ENST00000674125.1 | 1167 | No protein | Retained intron | - | - | - | - |
| MLH1-225 | ENST00000673686.1 | 1127 | No protein | Retained intron | - | - | - | - |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | MLH1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene MLH1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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