CRISPR-U™ Gene Knockout Cell Line
Strategy
PRDX4 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human PRDX4 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | PRDX4 |
| Gene id | 10549 |
| Organism | Homo sapiens |
| Official full symbol | peroxiredoxin 4 |
| Gene type | protein-coding |
| Also known as | AOE37-2, AOE372, HEL-S-97n, PRX-4 |
| Summary | The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. |
| Genomic regions | Chromosome X |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| PRDX4-202 | ENST00000379341.9 | 956 | 271aa | Protein coding | CCDS14206 | Q13162 V9HW63 | NM_006406.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| PRDX4-201 | ENST00000379331.3 | 852 | 186aa | Protein coding | - | A6NG45 | - | TSL:2, GENCODE basic, |
| PRDX4-203 | ENST00000379349.5 | 815 | 185aa | Protein coding | - | A6NJJ0 | - | CDS 3' incomplete, TSL:3, |
| PRDX4-204 | ENST00000439422.1 | 554 | 161aa | Protein coding | - | H7C3T4 | - | CDS 5' incomplete, TSL:3, |
| PRDX4-205 | ENST00000495599.1 | 632 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | PRDX4 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene PRDX4 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: