CRISPR-U™ Gene Knockout Cell Line Strategy

PRDX4 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human PRDX4 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol PRDX4
Gene id 10549
Organism Homo sapiens
Official full symbol peroxiredoxin 4
Gene type protein-coding
Also known as AOE37-2, AOE372, HEL-S-97n, PRX-4
Summary The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Genomic regions Chromosome X
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
PRDX4-202 ENST00000379341.9 956 271aa Protein coding CCDS14206 Q13162 V9HW63 NM_006406.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
PRDX4-201 ENST00000379331.3 852 186aa Protein coding - A6NG45 - TSL:2, GENCODE basic,
PRDX4-203 ENST00000379349.5 815 185aa Protein coding - A6NJJ0 - CDS 3' incomplete, TSL:3,
PRDX4-204 ENST00000439422.1 554 161aa Protein coding - H7C3T4 - CDS 5' incomplete, TSL:3,
PRDX4-205 ENST00000495599.1 632 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene PRDX4
This KO Strategy loading
Red Cotton™ Notes Gene PRDX4 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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