CRISPR-U™ Gene Knockout Cell Line Strategy

KRT8 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human KRT8 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol KRT8
Gene id 3856
Organism Homo sapiens
Official full symbol keratin 8
Gene type protein-coding
Also known as CARD2, CK-8, CK8, CYK8, K2C8, K8, KO
Summary This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene.
Genomic regions Chromosome 12
Strategy Summary
This gene has 9 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
KRT8-216 ENST00000552551.5 2126 483aa Protein coding CCDS8841 P05787-1 - TSL:2, GENCODE basic, APPRIS P2,
KRT8-201 ENST00000293308.11 1882 483aa Protein coding CCDS8841 P05787-1 - TSL:5, GENCODE basic, APPRIS P2,
KRT8-205 ENST00000546897.5 1755 483aa Protein coding CCDS8841 P05787-1 - TSL:2, GENCODE basic, APPRIS P2,
KRT8-215 ENST00000552150.5 1687 511aa Protein coding CCDS58234 P05787-2 - TSL:1, GENCODE basic, APPRIS ALT2,
KRT8-217 ENST00000619952.2 2437 32aa Protein coding - A0A0U1RQU6 - TSL:2, GENCODE basic,
KRT8-204 ENST00000546826.5 1165 273aa Protein coding - F8VUG2 - CDS 3' incomplete, TSL:5,
KRT8-210 ENST00000548998.5 918 289aa Protein coding - F8W1U3 - CDS 3' incomplete, TSL:5,
KRT8-202 ENST00000546542.1 765 180aa Protein coding - F8VQY3 - CDS 3' incomplete, TSL:3,
KRT8-206 ENST00000546900.1 523 113aa Protein coding - F8VP67 - CDS 3' incomplete, TSL:3,
KRT8-209 ENST00000547176.3 250 35aa Nonsense mediated decay - H0YIB2 - CDS 5' incomplete, TSL:5,
KRT8-212 ENST00000549198.1 592 No protein Processed transcript - - - TSL:2,
KRT8-214 ENST00000551318.1 507 No protein Processed transcript - - - TSL:2,
KRT8-203 ENST00000546583.5 2862 No protein Retained intron - - - TSL:5,
KRT8-213 ENST00000550170.5 2202 No protein Retained intron - - - TSL:5,
KRT8-208 ENST00000547031.5 883 No protein Retained intron - - - TSL:2,
KRT8-211 ENST00000549176.1 770 No protein Retained intron - - - TSL:2,
KRT8-207 ENST00000546921.1 542 No protein Retained intron - - - TSL:4,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene KRT8
This KO Strategy loading
Red Cotton™ Notes Gene KRT8 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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