CRISPR-U™ Gene Knockout Cell Line
Strategy
KRT8 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human KRT8 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | KRT8 |
| Gene id | 3856 |
| Organism | Homo sapiens |
| Official full symbol | keratin 8 |
| Gene type | protein-coding |
| Also known as | CARD2, CK-8, CK8, CYK8, K2C8, K8, KO |
| Summary | This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 9 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| KRT8-216 | ENST00000552551.5 | 2126 | 483aa | Protein coding | CCDS8841 | P05787-1 | - | TSL:2, GENCODE basic, APPRIS P2, |
| KRT8-201 | ENST00000293308.11 | 1882 | 483aa | Protein coding | CCDS8841 | P05787-1 | - | TSL:5, GENCODE basic, APPRIS P2, |
| KRT8-205 | ENST00000546897.5 | 1755 | 483aa | Protein coding | CCDS8841 | P05787-1 | - | TSL:2, GENCODE basic, APPRIS P2, |
| KRT8-215 | ENST00000552150.5 | 1687 | 511aa | Protein coding | CCDS58234 | P05787-2 | - | TSL:1, GENCODE basic, APPRIS ALT2, |
| KRT8-217 | ENST00000619952.2 | 2437 | 32aa | Protein coding | - | A0A0U1RQU6 | - | TSL:2, GENCODE basic, |
| KRT8-204 | ENST00000546826.5 | 1165 | 273aa | Protein coding | - | F8VUG2 | - | CDS 3' incomplete, TSL:5, |
| KRT8-210 | ENST00000548998.5 | 918 | 289aa | Protein coding | - | F8W1U3 | - | CDS 3' incomplete, TSL:5, |
| KRT8-202 | ENST00000546542.1 | 765 | 180aa | Protein coding | - | F8VQY3 | - | CDS 3' incomplete, TSL:3, |
| KRT8-206 | ENST00000546900.1 | 523 | 113aa | Protein coding | - | F8VP67 | - | CDS 3' incomplete, TSL:3, |
| KRT8-209 | ENST00000547176.3 | 250 | 35aa | Nonsense mediated decay | - | H0YIB2 | - | CDS 5' incomplete, TSL:5, |
| KRT8-212 | ENST00000549198.1 | 592 | No protein | Processed transcript | - | - | - | TSL:2, |
| KRT8-214 | ENST00000551318.1 | 507 | No protein | Processed transcript | - | - | - | TSL:2, |
| KRT8-203 | ENST00000546583.5 | 2862 | No protein | Retained intron | - | - | - | TSL:5, |
| KRT8-213 | ENST00000550170.5 | 2202 | No protein | Retained intron | - | - | - | TSL:5, |
| KRT8-208 | ENST00000547031.5 | 883 | No protein | Retained intron | - | - | - | TSL:2, |
| KRT8-211 | ENST00000549176.1 | 770 | No protein | Retained intron | - | - | - | TSL:2, |
| KRT8-207 | ENST00000546921.1 | 542 | No protein | Retained intron | - | - | - | TSL:4, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | KRT8 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene KRT8 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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