CRISPR-U™ Gene Knockout Cell Line
Strategy
ERP29 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human ERP29 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | ERP29 |
| Gene id | 10961 |
| Organism | Homo sapiens |
| Official full symbol | endoplasmic reticulum protein 29 |
| Gene type | protein-coding |
| Also known as | C12orf8, ERp28, ERp31, HEL-S-107, PDI-DB, PDIA9 |
| Summary | This gene encodes a protein which localizes to the lumen of the endoplasmic reticulum (ER). It is a member of the protein disulfide isomerase (PDI) protein family but lacks an active thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase. The canonical protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Genomic regions | Chromosome 12 |
Strategy Summary
This gene has 4 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| ERP29-201 | ENST00000261735.4 | 1623 | 261aa | Protein coding | CCDS9158 | P30040-1 | NM_006817.4 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| ERP29-202 | ENST00000455836.1 | 1333 | 53aa | Protein coding | CCDS44977 | P30040-2 | - | TSL:2, GENCODE basic, |
| ERP29-203 | ENST00000546477.1 | 1698 | 160aa | Protein coding | - | F8VY02 | - | TSL:3, GENCODE basic, |
| ERP29-204 | ENST00000552052.1 | 284 | 95aa | Protein coding | - | F8W1G0 | - | CDS 5' and 3' incomplete, TSL:3, |
| ERP29-205 | ENST00000553161.1 | 916 | No protein | Retained intron | - | - | - | TSL:1, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | ERP29 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene ERP29 had been KO in hek293t cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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