CRISPR-U™ Gene Knockout Cell Line Strategy

ERP29 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human ERP29 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol ERP29
Gene id 10961
Organism Homo sapiens
Official full symbol endoplasmic reticulum protein 29
Gene type protein-coding
Also known as C12orf8, ERp28, ERp31, HEL-S-107, PDI-DB, PDIA9
Summary This gene encodes a protein which localizes to the lumen of the endoplasmic reticulum (ER). It is a member of the protein disulfide isomerase (PDI) protein family but lacks an active thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase. The canonical protein dimerizes and is thought to play a role in the processing of secretory proteins within the ER. Alternative splicing results in multiple transcript variants encoding different isoforms.
Genomic regions Chromosome 12
Strategy Summary
This gene has 4 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
ERP29-201 ENST00000261735.4 1623 261aa Protein coding CCDS9158 P30040-1 NM_006817.4 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
ERP29-202 ENST00000455836.1 1333 53aa Protein coding CCDS44977 P30040-2 - TSL:2, GENCODE basic,
ERP29-203 ENST00000546477.1 1698 160aa Protein coding - F8VY02 - TSL:3, GENCODE basic,
ERP29-204 ENST00000552052.1 284 95aa Protein coding - F8W1G0 - CDS 5' and 3' incomplete, TSL:3,
ERP29-205 ENST00000553161.1 916 No protein Retained intron - - - TSL:1,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene ERP29
This KO Strategy loading
Red Cotton™ Notes Gene ERP29 had been KO in hek293t cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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