CRISPR-U™ Gene Knockout Cell Line Strategy

IPO9 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human IPO9 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol IPO9
Gene id 55705
Organism Homo sapiens
Official full symbol importin 9
Gene type protein-coding
Also known as Imp9
Genomic regions Chromosome 1
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
IPO9-201 ENST00000361565.9 11416 1041aa Protein coding CCDS1415 Q96P70 NM_018085.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
IPO9-202 ENST00000456707.1 609 155aa Protein coding - Q5SVH5 - CDS 5' incomplete, TSL:3,
IPO9-203 ENST00000464348.5 838 No protein Processed transcript - - - TSL:5,
IPO9-204 ENST00000479374.1 704 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
Strategy
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene IPO9
This KO Strategy loading
Red Cotton™ Notes Gene IPO9 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:

$49

Single gRNA plasmid off-shelf

$599

Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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