CRISPR-U™ Gene Knockout Cell Line
Strategy
FABP5 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FABP5 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FABP5 |
| Gene id | 2171 |
| Organism | Homo sapiens |
| Official full symbol | fatty acid binding protein 5 |
| Gene type | protein-coding |
| Also known as | E-FABP, EFABP, KFABP, PA-FABP, PAFABP |
| Summary | This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs may play roles in fatty acid uptake, transport, and metabolism. Polymorphisms in this gene are associated with type 2 diabetes. The human genome contains many pseudogenes similar to this locus. |
| Genomic regions | Chromosome 8 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FABP5-201 | ENST00000297258.11 | 676 | 135aa | Protein coding | CCDS6228 | E7DVW5 Q01469 | NM_001444.3 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FABP5-202 | ENST00000396359.1 | 986 | 101aa | Protein coding | - | I6L8B7 | - | TSL:5, GENCODE basic, |
| FABP5-203 | ENST00000481695.1 | 756 | No protein | Retained intron | - | - | - | TSL:2, |
| FABP5-204 | ENST00000486269.1 | 579 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FABP5 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FABP5 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
Comment: