CRISPR-U™ Gene Knockout Cell Line Strategy

FOXA1 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human FOXA1 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol FOXA1
Gene id 3169
Organism Homo sapiens
Official full symbol forkhead box A1
Gene type protein-coding
Also known as HNF3A, TCF3A
Summary This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.
Genomic regions Chromosome 14
Strategy Summary
This gene has 1 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
FOXA1-201 ENST00000250448.5 3509 472aa Protein coding CCDS9665 P55317-1 NM_004496.5 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
FOXA1-203 ENST00000553751.1 507 36aa Nonsense mediated decay - G3V4B9 - TSL:4,
FOXA1-202 ENST00000545425.2 1806 No protein Processed transcript - - - TSL:2,
FOXA1-204 ENST00000554607.1 789 No protein Processed transcript - - - TSL:3,
FOXA1-205 ENST00000557418.1 392 No protein Processed transcript - - - TSL:3,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene FOXA1
This KO Strategy loading
Red Cotton™ Notes Gene FOXA1 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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