CRISPR-U™ Gene Knockout Cell Line
Strategy
FOXA1 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human FOXA1 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | FOXA1 |
| Gene id | 3169 |
| Organism | Homo sapiens |
| Official full symbol | forkhead box A1 |
| Gene type | protein-coding |
| Also known as | HNF3A, TCF3A |
| Summary | This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. |
| Genomic regions | Chromosome 14 |
Strategy Summary
This gene has 1 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| FOXA1-201 | ENST00000250448.5 | 3509 | 472aa | Protein coding | CCDS9665 | P55317-1 | NM_004496.5 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| FOXA1-203 | ENST00000553751.1 | 507 | 36aa | Nonsense mediated decay | - | G3V4B9 | - | TSL:4, |
| FOXA1-202 | ENST00000545425.2 | 1806 | No protein | Processed transcript | - | - | - | TSL:2, |
| FOXA1-204 | ENST00000554607.1 | 789 | No protein | Processed transcript | - | - | - | TSL:3, |
| FOXA1-205 | ENST00000557418.1 | 392 | No protein | Processed transcript | - | - | - | TSL:3, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | FOXA1 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene FOXA1 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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