CRISPR-U™ Gene Knockout Cell Line
Strategy
CDK6 Gene Knockout Strategy
CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general
CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has
successfully edited over 3000 genes on more than 100 types of cell lines.
Objective
To create a Human CDK6 Knockout
model in cell line by
CRISPR-U™-mediated
genome
engineering.
Target gene info 
| Official symbol | CDK6 |
| Gene id | 1021 |
| Organism | Homo sapiens |
| Official full symbol | cyclin dependent kinase 6 |
| Gene type | protein-coding |
| Also known as | MCPH12, PLSTIRE |
| Summary | The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly. |
| Genomic regions | Chromosome 7 |
Strategy Summary
This gene has 2 protein coding transcripts：
| Name | Transcript ID | bp | Protein | Biotype | CCDS | UniProt Match | RefSeq Match | Flags |
| CDK6-202 | ENST00000424848.3 | 11663 | 326aa | Protein coding | CCDS5628 | Q00534 | NM_001145306.2 | TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92, |
| CDK6-201 | ENST00000265734.8 | 11612 | 326aa | Protein coding | CCDS5628 | Q00534 | - | TSL:1, GENCODE basic, APPRIS P1, |
| CDK6-205 | ENST00000491250.1 | 558 | No protein | Processed transcript | - | - | - | TSL:4, |
| CDK6-203 | ENST00000467166.1 | 779 | No protein | Retained intron | - | - | - | TSL:2, |
| CDK6-204 | ENST00000473078.1 | 386 | No protein | Retained intron | - | - | - | TSL:2, |
Red Cotton™ Assessment
Project Difficulty Level unknown
| Target Gene | CDK6 |
| This KO Strategy | loading |
| Red Cotton™ Notes | Gene CDK6 had been KO in hela cell line. |
Aforementioned information comes from Ubigene database. Different origin of cell lines may have
different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:
$49
Single gRNA plasmid off-shelf
$599
Single gRNA lentivirus
Work flow
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