CRISPR-U™ Gene Knockout Cell Line Strategy

CDK6 Gene Knockout Strategy

CRISPR-U™ technology (CRISPR based), developed by Ubigene, is more efficient than general CRISPR/Cas9 technology in double-strand breaking and homologous recombination. With CRISPR-U™, Ubigene has successfully edited over 3000 genes on more than 100 types of cell lines.
To create a Human CDK6 Knockout model in cell line by CRISPR-U™-mediated genome engineering.
Target gene info
Official symbol CDK6
Gene id 1021
Organism Homo sapiens
Official full symbol cyclin dependent kinase 6
Gene type protein-coding
Also known as MCPH12, PLSTIRE
Summary The protein encoded by this gene is a member of the CMGC family of serine/threonine protein kinases. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Altered expression of this gene has been observed in multiple human cancers. A mutation in this gene resulting in reduced cell proliferation, and impaired cell motility and polarity, and has been identified in patients with primary microcephaly.
Genomic regions Chromosome 7
Strategy Summary
This gene has 2 protein coding transcripts:
Name Transcript ID bp Protein Biotype CCDS UniProt Match RefSeq Match Flags
CDK6-202 ENST00000424848.3 11663 326aa Protein coding CCDS5628 Q00534 NM_001145306.2 TSL:1, GENCODE basic, APPRIS P1, MANE Select v0.92,
CDK6-201 ENST00000265734.8 11612 326aa Protein coding CCDS5628 Q00534 - TSL:1, GENCODE basic, APPRIS P1,
CDK6-205 ENST00000491250.1 558 No protein Processed transcript - - - TSL:4,
CDK6-203 ENST00000467166.1 779 No protein Retained intron - - - TSL:2,
CDK6-204 ENST00000473078.1 386 No protein Retained intron - - - TSL:2,
Ubigene Red Cotton Transcript
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Red Cotton™ Assessment    
Project Difficulty Level unknown
Target Gene CDK6
This KO Strategy loading
Red Cotton™ Notes Gene CDK6 had been KO in hela cell line.
Aforementioned information comes from Ubigene database. Different origin of cell lines may have different condition. Ubigene reserved all the right for final explanation.
Special deals for this gene:


Single gRNA plasmid off-shelf


Single gRNA lentivirus

Work flow
Ubigene Red Cotton Workflow

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